Designing an Efficient Multi-epitopeVaccine against Acinetobacter baumanniiusing Immunoinformatics Approaches
- سال انتشار: 1398
- محل انتشار: سیزدهمین کنگره بین المللی میکروب شناسی بالینی استاد البرزی
- کد COI اختصاصی: ICCM13_063
- زبان مقاله: انگلیسی
- تعداد مشاهده: 430
نویسندگان
Food Health Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Food Health Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
چکیده
Background and Objectives: Acinetobacter baumannii (A. baumannii) is an emerging multi-drug resistant pathogen causing significant mortality inhospitalized ICU patients. The emergence of antibiotic resistance has increased mortality and costs of treatment. The design of a vaccine against A. baumannii would have a great beneficial impact on public health. Materials and Methods: Three known antigenic proteins from A. baumannii ( OmpA,Phospholipase D and Bap ) were analyzed for the prediction of immunogenic B cell and Tcell epitopes, using online algorithms. Based on in silico analysis, epitope-rich domains including highly scored T cell and B cell epitope were selected and designed protein validated using the ProSA-web, Molprobity and the Ramachandran plot. The obtained highquality model was applied for conformational B-cell epitopes selection and docking analyses with toll like receptor 4 (TLR4). The affinity and the map of interactions between the receptor (TLR4) and recombinant protein were investigated via docking protocols. Result: Molecular dynamic studies demonstrated that the recombinant protein-TLR4 docked model was stable during simulation time. We believe that our vaccine candidate is able to induce IgG antibodies, and Th1/Th17-mediated protective immunity that are crucial for eradicating A. baumannii infection. Conclusion: The in silico analysis showed that the designed recombinant protein ( OmpA,Phospholipase D and Bap ) could be considered as apromising vaccine candidate against A. baumannii.کلیدواژه ها
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