Proliferative Effect of FadA Recombinant Protein from Fusobacteriumnucleatum on SW480 Colorectal Cancer Cell Line
- سال انتشار: 1398
- محل انتشار: سیزدهمین کنگره بین المللی میکروب شناسی بالینی استاد البرزی
- کد COI اختصاصی: ICCM13_057
- زبان مقاله: انگلیسی
- تعداد مشاهده: 608
نویسندگان
Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj Iran
Department of Microbiology, School of Medicine, ShahidBeheshti University of Medical Sciences, Tehran, Iran
Molecular Microbiology Research Center, Shahed University, Tehran, Iran
Department of Microbiology, School of Medicine, ShahidBeheshti University of Medical Sciences, Tehran, Iran
چکیده
Background and Objetives: Colorectal Cancer (CRC) is one of the most frequent cancers diagnosed in both men and women worldwide. Fusobacteriumnucleatumadhesin A (FadA) has an important potential factor in the development or progression of CRC. The aim of the present study was to evaluate the proliferative effect of recombinant FadA on SW480 colorectal cancer cell line. Materials and Methods: The recombinant pET21 (b)-fadA plasmid was synthesized and transformed into competent E.coli DH5α. In the next step, induction and expression of recombinant FadA were carried out in E. coli BL21 (DE3) competent cells. Expression and purification of protein were successfully done and it was analyzed and confirmed by SDS-PAGE and western blotting. The proliferative effect of purified FadA on SW480 CRC cell line was evaluated using MTT assay and cell counting methods. Results: Visualization of the specific band isolated from the linear plasmid on the agarose gel confirmed the presence of the desired gene. After electrophoresis and Coomassie blue staining, the protein of interest with an approximate molecular weight of 13KDa was detected. The MTT assay, similar to cell counting methods, revealed that FadA dose and time-dependently promoted SW480 cell growth and proliferation in 24, 48 and 72 hours. Conclusion: The results showed that FadA stimulates proliferation of SW480 colorectal cancer cell line with a dose and time-dependent manner.کلیدواژه ها
مقالات مرتبط جدید
- دانش سنتی در مدیریت و بهرهبرداری قنات مزویرآباد، مهریز، یزد
- Inhibitory Activity of Oliveria decumbens Essential Oil on Staphylococcus aureus Biofilm and Planktonic Cells
- Evaluation of Anti-leishmanial Effect of Selenium Nanoparticles on Leishmania major Promastigotes In Vitro
- Prevalence and Molecular Mapping of Multidrug-resistant Mycobacterium tuberculosis in Sokoto, North-Western Nigeria
- Viral Gastroenteritis Prevalence in Iranian Pediatric Population: A Systematic Review
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.