Comparison between Acute and Recurrent Urinary Tract Infection according to the Distribution of Virulence Factors within Escherichia coli Strains Isolated in both Phases
- سال انتشار: 1398
- محل انتشار: سیزدهمین کنگره بین المللی میکروب شناسی بالینی استاد البرزی
- کد COI اختصاصی: ICCM13_018
- زبان مقاله: انگلیسی
- تعداد مشاهده: 440
نویسندگان
Biology Department, Islamic Azad University Tehran North Branch
Biology Department, Islamic Azad University Science and Research Branch
Department of Medical Engineering, Faculty of Engineering, University of Malasia Kuala Lumpur, Malaysia
Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences
چکیده
Background: Treatment and prevention of hepatitis C virus (HCV) infections remain a major challenge for controlling this worldwide health problem controlling this worldwide health problem. Methodes and Matherials: In this study, we investigated the role of let-7b that were overexpressed by lentiviral vector against highly conserved HCV-1 sequences. Since the main target of this microRNA is the NS3 gene,it was amplified, cloned into ptz57R/T cloning vestor and then subcloned into psicheck-2, respectively. In order to doing Luciferase assay test, lentiviral vector-let-7b and moc were transduced into Huh-7.5 as a hepatocyte origine cells and then schicheck-2 constructs were transformed into them. Results: Follwing performance of a luciferase assay, the specific binding of this microRNA to the viral genome was confirmed. Conclusions:This data suggest that let-7b can downregulate of HCV proliferation in Huh-7.5 cell lines.کلیدواژه ها
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