Graphene- based nano composites: Synthesis, Characterization, and biological applications

Graphene- based nano composites: Synthesis, Characterization, and biological applications
  • سال انتشار: 1397
  • محل انتشار: پانزدهمین همایش بیوشیمی فیزیک ایران
  • کد COI اختصاصی: CBC15_014
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 499
دانلود فایل این مقاله

نویسندگان

Asghar Taheri-Kafrani

Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, ۸۱۷۴۶-۷۳۴۴۱, Iran

Hadi Zare-Zardini

Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, ۸۱۷۴۶-۷۳۴۴۱, Iran

چکیده

In this study, Rh2–treated graphene oxide (GO-Rh2), lysine-treated highly porous graphene oxide nanosheets (GO-Lys), arginine-treated GO (GO-Arg), Rh2–treated GO-Lys (GO-Lys-Rh2) and Rh2–treated GO-Arg (GO-Arg-Rh2) were synthesized. MTT assay was used for evaluation of cytotoxicity of samples on ovarian cancer (OVCAR3), breast cancer (MDA-MB), Human melanoma (A375) and human mesenchymal stem cells (MSCs) cell lines. The percentage of apoptotic cells was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. The hemolysis and blood coagulation activity of nanostructures were performed. Interestingly, GO-Arg, GO-Lys, GO-Arg-Rh2, and GO-Lys-Rh2 were more active against cancer cell lines in comparison with their cytotoxic activity against normal cell lines (MSCs) with IC50 values higher than 100 μg/ml. The results of TUNEL assay indicates a significant increase in the rates of TUNEL positive cells by increasing the concentrations of nanomaterials. Results were also shown that aggregation and changes of RBCs morphology were occurred in the presence of GO, GO-Rh2, GO-Arg, GO-Lys, GO-Arg-Rh2, and GO-Lys-Rh2. Note that all the samples had effect on blood coagulation system, especially on PTT. All nanostrucure act as antitumor drug so that binding of drugs to a nostructures is irresolvable and the whole structure enter to the cell as a drug.

کلیدواژه ها

Anti-cancer activity, Ginsenoside Rh2, Graphene Oxide, Drug delivery, Hemolysis, Coagulation

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.