Study and evaluation of antibacterial properties of clove essential oil
- سال انتشار: 1397
- محل انتشار: بیستمین کنگره ملی و هشتمین کنگره بین‌المللی زیست‌شناسی ایران
- کد COI اختصاصی: BIOCONF20_563
- زبان مقاله: انگلیسی
- تعداد مشاهده: 305
نویسندگان
Faculty of Chemical Engineering, Sahand University of Technology, East Azarbaijan, Tabriz, Iran
Faculty of Chemical Engineering, Sahand University of Technology, East Azarbaijan, Tabriz, Iran. Research and Development Department, Najian Herbal Group, East Azarbaijan, Tabriz, Iran
Research and Development Department, Najian Herbal Group, East Azarbaijan, Tabriz, Iran
Research and Development Department, Najian Herbal Group, East Azarbaijan, Tabriz, Iran
چکیده
Medicinal herbs have long been used traditionally for treatment of human diseases and recent scientific and technology advances have made much more importance and role of medicinal herbs in meeting human needs, especially in the field of medicine and treatment. These include the role of medicinal plants in the treatment and prevention of gum disease and dental caries. Streptococcus mutans is known as one of the most important etiologic factors in plaque formation and tooth decay. Streptococcus mutans is a spherical gram-positive bacterium, have a capsule, produce acid and remain stable at the mucosal surfaces exposed to saliva flow, with colony formation and replication. Clove as an important plant is an effective herb in ancient and modern medicine, and the essential oil of Clove has a significant anti-bacterial effect. In this study, the effect of Clove on Streptococcus mutans, dental caries was investigated. So that the distillate and essential oil of dried Clove blooms were prepared using a clevenger apparatus through distillation with water and steam. Antimicrobial activity of Clove distillate and essential oil against Streptococcus mutans was investigated using an antimicrobial laboratory method of agar well diffusion method. In this method, plate surface containing nutrient agar is inoculated by spreading a volume of the microbial inoculum over the entire agar surface. Then, a hole with a diameter of 6 mm is punched aseptically using gel puncher and a volume (20 μL) of the antimicrobial agent solution is introduced into the well. Then, agar plates are incubated at 37°C for 24h. The antimicrobial agent diffuses in the agar medium and inhibits the growth of the microbial strain tested. Based on the results, the distillate from Clove flowers did not have an antibacterial effect on Streptococcus mutans. In contrast, Clove essential oil showed a significant effect on inhibiting the growth of the bacteria and the inhibition zones in some repetitions was obtained about 22 to 31 mm in average.کلیدواژه ها
Clove, essential oil, antibacterial property, inhibition zoneمقالات مرتبط جدید
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