Antibiotic resistance and prevalence of vim-1,vim-2, sim and ndm genes in non-infectious children under 3 years old in Khuzestan province

سال انتشار: 1397
محل انتشار: دوازدهمین کنگره بین المللی میکروب شناسی بالینی ایران
کد COI اختصاصی: ICCM12_094
زبان مقاله: انگلیسی
تعداد مشاهده: 546

نویسندگان

Biology Department; Faculty of Science; Shahid Chamran University of Ahvaz; Ahvaz; Iran

چکیده

Introduction and Objective: Escherichia coli is widely found in flora of gastrointestinal tract and can be involved in infectious diseases of humans. Microorganisms of flora may have resistance genes that can be transmitted from the commensal flora to pathogenic microorganisms. The dispersion of gram-negative bacteria producing metallo-beta-lactamase (MBL) is increasing. The aim of the present study was investigation of the antimicrobial resistance and prevalence of vim-1, vim-2, sim and ndm genes among E. coli strains isolated from feces of non-infectious children under three years old in Khuzestan province.Materials and Methods: 130 anal swabs were taken from non-infectious children. These specimens were examined after culture in a dedicated culture medium. Kirby-Bauer disk diffusion method was performed to analyze antimicrobial resistance among E. coli isolates from commensal flora. Resistance pattern of 6 antibiotics including ampicillin, kanamycin, ceftazidime, trimethoprim-sulfamethoxazole, ciprofloxacin, and imipenem was investigated according to CLSI 2016. To determine MBL-producing isolates, imipenem resistance strains was evaluated by PCR method for presence of vim-1, vim-2, sim and ndm genes. Result: The highest resistance was observed against ampicillin (56.92%). The percent of resistance related to kanamycin, ceftazidime, trimethoprim-sulfamethoxazole, ciprofloxacin, and imipenem was as follow: (5/38%), (30/77%), (41/54), (14/61) and (3/08) respectively. The genes of ndm and vim-2 were found in 2 isolates; while none of isolates harbored vim-1 and sim genes.118Conclusion: Study showed the high frequency of antimicrobial resistance among commensal isolates; therefore, is needed to control the use of antimicrobial agents among people. Increasing of antibiotic resistance can lead to treatment failure in countries with high antibiotic use.

کلیدواژه ها

commensal Escherichia coli, MBL, antibiotic resistance, disc diffusion method

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