The bench of Hair follicle stem cell transplantation in cutaneous wound healing of Rat
- سال انتشار: 1397
- محل انتشار: نوزدهمین کنگره پژوهشی سالانه دانشجویان علوم پزشکی کشور
- کد COI اختصاصی: AMSMED19_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 466
نویسندگان
Student Research Committee, Qom University of Medical Science, Qom, Iran
Assistant Prof, Department of Anatomical Sciences, Qom University of Medical Science, Qom, Iran
Student Research Committee, Qom University of Medical Science, Qom, Iran
Student Research Committee, Qom University of Medical Science, Qom, Iran
چکیده
Background and Objective: Nowadays skin wound healing become a serious clinical problem especially after surgery and sever injury of skin, because it increases the risk of injury site infections, postoperative hospitalizations, and rises medical expenses; so there is an urgent requirement to develop innovative techniques in the case of wound healing such as adult stem cell therapy. Materials and Methods: Animals (male Wistar rats) were divided into 3 groups: 1) Control (non-treated), 2) Vehicle (PBS), 3) HFSCs (treated with HFSCs). Briefly, after anesthesia, the dorsum skin of rats was shaved, and cleaned with betadine. Then full thickness excisional wounds were created using biopsy punch. The Bulge region of rat whiskers was isolated and cultured in DMEM/F12, then Wound healing was recorded with digital photographs. At the end of the treatment period, histological assessments (H&E and Masson s trichrome staining) and molecular assays (ELISA and RT- PCR) for VEGF, SDF-1a and CXCR4 were performed.Findings: Morphological analysis of wounds exhibited wound closure in HFSC treated group. Histological analysis of H&E and Masson s trichrome stained photomicrographs showed much re-epithelialization and dermal structural regeneration properly. In fact, the diameter of epidermis and wound healing percent in HFSCs group were significant compared with control group (P< 0.05). in molecular assays, angiogenesis (VEGF level), SDF-1a and CXCR4 expression and protein secretion in HFSCs groups were more significant compare with control group (P< 0.05). Conclusion: Current therapies including wound dressings, surgery, topical negative pressure or skin substitutes are not impressible methods and there is an urgent requirement to develop innovative techniques such as cell therapy in the case of wound healing. Adult stem cells such as HFSC, obtained from the skin and might become an accessible source for autologous cell-replacement therapy; Transplantation of HFSCs, induce secretion of SDF-1 and CXCR4 expression in wound bed which play important role in angiogenesis and accelerate cutaneous wound healing. In gist transplantation of hair follicle stem cells had potential capability for cutaneous wound healing.کلیدواژه ها
Wound healing, HFSC, SDF-1, CXCR4, ELISA, RT- PCRمقالات مرتبط جدید
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