EVALUATION OF THE PRODUCTION RATE OF EXTENDED-SPECTRUM BETA-LACTAMASE ENZYMES BY GRAM-NEGATIVE BACTERIA IN DIFFERENT CLINICAL SAMPLES ISOLATED FROM PATIENTS
- سال انتشار: 1397
- محل انتشار: نوزدهمین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM19_092
- زبان مقاله: انگلیسی
- تعداد مشاهده: 470
نویسندگان
Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran. Cellular &Molecular Research Center, Kurdistan University of Medical, Sanandaj, Iran
Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
Toohid Hospital, Kurdistan University of Medical Sciences, Sanandaj, Iran
Cellular &Molecular Research Center, Kurdistan University of Medical, Sanandaj, Iran
چکیده
Background and Aim:Treatment of infections depends on the sensitivity of these bacteria to antibiotic drugs. Therefore, the aim of this study was to detect the production rate of ESBL enzymes by bacteria from clinical samples isolated from patients.Methods:This descriptive- cross-sectional study was conducted during a one-month period in March, 2017. The study population included all clinical samples isolated from Toohid Medical Center, Sanandaj, Kurdistan province, Iran.. Combined disk method using 30µg of ceftoxime and cefotaxime30µg/clavulanic acid 10 µg was used to detect strains producing ESBL enzymes in different bacteria. According to the CLSI, if the growth halo around the cefotaxime/ clavulanic acid was ≥5 mm apart than cefotaxime alone, the strain is considered to be an enzyme producer. Data were analyzed by SPSS16 and frequency and ANOVA method (p< 0.05).Results:Thirty seven clinical specimens, including bacteria producing ESBL enzymes, were observed. Of these, 31 (83.78%) were urinary culture. The bacteria producing the enzyme isolated from urine culture included 5 Klebsiella genus (16.12%) and 26 (83.87%) of Escherichia coli strains. Of 4 cases of lung secretion, 3 (75%) Klebsiella genus and 1 (25%) Acinetobacter genus was isolate. Of the two collected ulcers specimens, all 2 (100%) samples, including Klebsiella genus and producing ESBL. Also 6/37 (16.21%) samples were isolated from patients with nosocomial infections. There was no significant relationship between the production of this enzyme and the type of sample (p> 0.05).Conclusion:The prevalence ESBL-producing bacteria in patients with were observed. Appropriate methods to prevent of this problem should be considered.کلیدواژه ها
Extended-spectrum beta-lactamase, gram-negative bacteria, clinical samples, patientsاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.