PCR Detection of Cytomegalovirus in Women With Recurrent Abortions
- سال انتشار: 1397
- محل انتشار: چهارمین کنگره بین المللی تولیدمثل
- کد COI اختصاصی: ISERB04_109
- زبان مقاله: انگلیسی
- تعداد مشاهده: 626
نویسندگان
Department of Microbiology – shahr-e-Qods Branch ,Islamic Azad University azad university
Department of Microbiology – shahr-e-Qods Branch ,Islamic Azad University ,Iranian Gene Fanavar Institute (IGF),
Department of Microbiology – shahr-e-Qods Branch ,Islamic Azad University azad university
چکیده
Background: According to WHO reports, 43% of women and 30.7% of men suffer from infertility. Infectious agents are an important factor in sterility and infertility .Cytomegalovirus is one of the infectious agents that can affect infertility . Rapid diagnosis of this agent can be a pioneer in the treatment and prevention of which moleculartechniques can be used for diagnosis. Purpose and Importance of Research: The purpose of this study was to investigate the role of Cytomegalovirus in infertile women withrecurrent abortions, by PCR method. Methods: Research was conducted on 100 samples of vaginal discharge in women with recurrent abortions from Sarem Hospital inTehran. Samples DNA were extracted by boiling/DNG method and optimized PCR test was performed on samples DNA. The test was also examined in terms of limit of detection (LOD) and specificity. Results: In this study PCR test was optimized and 257bp PCR product was amplified by using of Cytomegalovirus specific primers. Specificity test showed primer 100% specificity.The limit of detection of PCR for CMV was 100 Copy/reaction. 5% of samples were positive by PCR Test.Discussion and Conclusion: This assessment demonstrates the role of Cytomegalovirus in infertility and the occurrence of repeated abortions, which, of course, requires further studies in this contextکلیدواژه ها
Cytomegalovirus, Infertility, Recurrent abortion, PCRاطلاعات بیشتر در مورد COI
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