Survey for protease activity in Cold-adapted indigenous bacteria

  • سال انتشار: 1395
  • محل انتشار: اولین کنفرانس ملی یافته های نوین زیست شناسی
  • کد COI اختصاصی: BIOC01_164
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 264
دانلود فایل این مقاله

نویسندگان

Reihaneh Nikbakhti

Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

Bahar Shahnavaz

Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

Ahmad Asoodeh

Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

چکیده

Cold-active proteases constitute an important group of these enzymes; have high-catalytic efficiencies at lower temperature and lower thermal stability. Numerous studies have indeed shown that cold-active proteases are gen-erally characterized by higher turnover numbers (kcat) and catalytic efficiencies (kcat/Km) at low temperatures(< 10 ◦C) compared to their mesophilic counterparts. Alkaline proteases from bacteria find numerous applications in various industrial sectors and different companies worldwide have successfully launched several products based on alkaline proteases. In this presence, protease producing bacteria was tested by halo zone in mineral salt agar containing skim milk. Enzyme production was determined using optical density. The enzyme production was measured in different temperatures (15, 20, 25, 30 and 37 °C). The impact of different nitrogen sources (yeast extract and skim milk) and concentrations (0.5, 1, 1.5 and 2%) was investigated. About 53% of the isolates showed a zone with a mean diameter of 2.14 mm, with 3 mm as the high diameter. The high enzyme production was obtained about 0.312 unit/ml. The highest level of protease production was obtained at 20 °C temperature (0/312 Uml-1). The protease production was increased by adding skim milk as a nitrogen source (0.594 Uml-1). The preliminary results showed that cold active indigenous bacteria are remarkable for enzyme production. These strains can potentially be used for the production of enzymes in various industries. Cold-adapted proteases thus can be use to optimize industrial processes with less energy inputs and process cost by removing the cost of heat inactivation step

کلیدواژه ها

protease, optimization,Skim Milk, Nitrogen

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.