Introduction of New Electrochemical Biosensor for Detection of DNA Hybridization
- سال انتشار: 1394
- محل انتشار: یازدهمین سمینار سالانه الکتروشیمی ایران
- کد COI اختصاصی: ELECTROCHEMISTRY011_340
- زبان مقاله: انگلیسی
- تعداد مشاهده: 521
نویسندگان
Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
چکیده
The development of DNA biosensors has recently attracted significant attentions in connection with research efforts directed at gene analysis, tissue matching, the detection of genetic disordersand forensic applications [1, 2]. To date, various sensing strategies for DNA detection such asfluorescence [3], quartz crystal microbalance [4], surface plasmon resonance spectroscopy , surface-enhanced Raman scattering and electrochemical have been employed. In this work, weintroduced a novel electrochemical DNA sensor for the detection of DNA hybridization. The new electrochemical biosensor was fabricated by modifying a screen printed graphite electrode (SPE) with gold nanoparticles decorated on graphene (AuNPs/G). The whole DNA biosensorfabrication process was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. The probe DNA immobilization and its hybridization on the modified electrode were further studied with CV and differential pulse voltammetry(DPV). Under optimum conditions, the developed biosensor showed a good linear relationship between the current value and logarithm of the target DNA concentration ranging from 0.5 fM to 0.05 nM with a detection limit of 3.64×10−16 M. Moreover the biosensor could effectively recognize the complementary target DNA from single base mismatched and non complementary target sequences.کلیدواژه ها
Screen printed electrode, Graphene nanosheets, DNA hybridization, and Differential pulse voltammetryمقالات مرتبط جدید
- Molecular Simulation of Water Transport through Polyamide Membrane in K+Cl- Salt Solution
- نانوکامپوزیت ها در دندانپزشکی
- Improving the characteristics of L-shaped graphene-based gas sensor using gold block on the surface
- Molecular Modelling of Gelatin Bundles for Atomistic Investigations Suggesting Two Stable Conformations
- بررسی تاثیر نانوذرات منیزیم بر جوانه زنی بذر و رشد گیاهچه بادرنجبویه Melissa officinalis
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.