Simultaneous determination of epinephrine and acetaminophen concentrations using a modified glassy carbon electrode prepared by Graphene and NiO nanoparticles

  • سال انتشار: 1394
  • محل انتشار: یازدهمین سمینار سالانه الکتروشیمی ایران
  • کد COI اختصاصی: ELECTROCHEMISTRY011_309
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 345
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نویسندگان

Mohammad Mazloum-Ardakani

Department of Chemistry, Faculty of Science, Yazd University, Islamic Republic of Iran

Fatemeh Farbod

Department of Chemistry, Faculty of Science, Yazd University, Islamic Republic of Iran

Laleh Hosseinzadeh

Department of Chemistry, Faculty of Science, Yazd University, Islamic Republic of Iran

چکیده

Based on the outstanding properties of graphene (GR) and nickel oxide nanoparticles (NiO NPs), modified electrode was prepared by casting GR solution and then electro depositionof NiO NPs on the glassy carbon electrode (GCE) surface. GR was obtained by chemical reduction of graphite oxide (GO) and characterized by scanning electron microscope and infrared spectroscopy. The electrochemical effective surface area of two modes of electrode including theNiO/GR/GCE and NiO/GCE were calculated 0.038 and 0.029 cm2 respectively. The electro catalytic properties of NiO/GR modified glassy carbon electrode toward the oxidation of epinephrine (EP) were analyzed by cyclic voltammetry (CV) and differential pulsevoltammetry (DPV). In CV studies, in the presence of EP, NiO/GR composite modifiedelectrode shows a significantly higher current response for epinephrine oxidation. At the pH of 7.0 in a 0.1M phosphate buffer solution, the DPV anodic peak currents showed a linear relationship versus EP concentrations in the range of 1.0–100μM and 100-1800.0 μM. The detection limit of 0.84 μM with the linear detection range from 1 μM to 100μM was obtained. The modified electrode exhibits an efficient electro catalytic behavior together with wellseparated oxidation peaks for EP and acetaminophen (AC). The diffusion coefficient (D =6.56×10-5 cm2 s–1), and the kinetic parameters such as electron transfer coefficient, (α = 0.33) andcatalytic rate constant, (k´ h = 8.7 cm s-1) for EP were also determined using electrochemical approaches. Finally, the resulting sensor was used to detect EP and AC simultaneously in bloodplasma samples. The results were explained using the theory of electro catalytic reactions at chemically modified electrodes

کلیدواژه ها

Graphene, Nickel oxide nanoparticles, Electro catalysis, Epinephrine, Acetaminophen

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