Detection of Methicillin Resistance in Staphylococcus aureus by Disk Diffusion and PCR Methods
- سال انتشار: 1387
- محل انتشار: فصلنامه آسیب شناسی ایران، دوره: 3، شماره: 1
- کد COI اختصاصی: JR_IJP-3-1_011
- زبان مقاله: انگلیسی
- تعداد مشاهده: 688
نویسندگان
Dept. of Microbiology, School of Medicine, Shahed University, Tehran, Iran
Dept. of Microbiology, School of Medicine, Shahed University, Tehran, Iran
Dept. of Microbiology, School of Medicine, Shahed University, Tehran, Iran
Dept. of Biotechnology, Pasteur Institute of Iran, Tehran, Iran
چکیده
Background and Objective: Methicillin resistance in Staphylococcus aureus is an increasingly important clinical problem. A chromosomal gene, mecA, mediates resistance to penicillinase-resistantpenicillins such as methicillin and oxacillin in Staphylococcus aureus. We evaluated the validityof disk diffusion test by using oxacillin, methicillin and cefoxitin disks with consideration of the presence of mecA gene as the reference method for detection of methicillin resistant Staphylococcusaureus (MRSA). Materials and Methods: The susceptibility testing of 222 S. aureus clinical isolates to oxacillin(1 μg), cefoxitin (30 μg) and methicillin (5 μg) was carried out by the disk diffusion method according to the Clinical Laboratory Standards Institute guidelines. Detection of mecA gene was performedusing PCR method. Results: An amplified mecA gene of 310 bp was detected in 55% of examined strains by PCR,thus 55% strains were considered MRSA. Sensitivity of oxacillin, methicillin and cefoxitin disks were determined 100%, 99.1% and 98.3% respectively.Conclusion: All MRSA strains in PCR had shown resistance to penicillinase-resistant penicillins by oxacillin disk, but two and one strains were sensitive by cefoxitin and methicillin disk respectively. Thus, oxacillin was the most appropriate disk for detecting MRSA. The prevalence of MRSA in thisstudy is comparable to that found in United States, Canada, Europe and Iran, but the percentage of MRSA isolates is almost twice of percentage reported from Japan.کلیدواژه ها
Staphylococcus aureus, PCR, mecA, Disk diffusionمقالات مرتبط جدید
- Effect of Salicylic Acid and Root Inoculation with P. indica on Cd Phytoremediation Efficiency and Degradation of Soil Fuel Oil in the Salinity Stress
- Acute Effect of Concurrent Exercise on Serum Leptin and Resistance Insulin Response in Sedentary Men
- Analysis of Incidents Recorded Data of in Kashan Fire Department During ۱۹۹۹-۲۰۱۶
- Development and Psychometric of “Public Awareness on Emergency Response in the Workplace Questionnaire”
- تجزیه و تحلیل علل ریشه ای حوادث شغلی در صنایع به کمک تکنیک های مرسوم بررسی حوادث مطالعه موردی یک حادثه منجر به فوت در پست ۲۳۰ کیلوولت
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.