Prevalence and Antibiotic Resistance Profiles of Metallo-β-lactamase Genes in Clinical Isolates of Pseudomonas aeruginosa

  • سال انتشار: 1403
  • محل انتشار: مجله بین المللی میکروبیولوژی مولکولی و بالینی، دوره: 14، شماره: 2
  • کد COI اختصاصی: JR_IJMCM-14-2_001
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 174
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نویسندگان

Mohammed Alduwayjee

Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Ashraf Kariminik

Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran, Food and Agricultural Safety Research Center, Kerman Branch, Islamic Azad University, Kerman, Iran

Atousa Ferdousi

Department of Microbiology, Shahr e Ghods Branch, Islamic Azad University, Tehran, Iran

چکیده

Pseudomonas aeruginosa is recognized as one of the most significant emerging threats in the current century. The production of metallo-β-lactamases is a critical mechanism of antibiotic resistance commonly associated with nosocomial infections caused by Pseudomonas aeruginosa. This study aimed to assess the antibiotic resistance profile and the prevalence of metallo-β-lactamase genes in clinical isolates of Pseudomonas aeruginosa. This cross-sectional study was conducted on ۱۰۰ Pseudomonas aeruginosa isolates obtained from patients at three hospitals in Tehran, Iran. Antibiotic susceptibility testing was performed using the standard Kirby-Bauer disk diffusion method. Additionally, the presence of specific metallo-β-lactamase genes, including bla-IMP, bla-VIM-۱, and bla-VIM-۲, were assessed through multiplex polymerase chain reaction. The isolates exhibited significant resistance to Amikacin (۹۸.۶%) and cefotaxime (۴۸.۹%). Imipenem was found to be the most effective antibiotic, showing a susceptibility rate of ۷۲.۶%. The genes bla-IMP, bla-VIM-۱, and bla-VIM-۲ were detected in ۳۴%, ۶۹%, and ۳۳% of the isolates, respectively. Furthermore, ۳۵% of the isolates carried all three genes: bla-IMP, bla-VIM-۱, and bla-VIM-۲. This study showed that multidrug-resistant isolates of Pseudomonas aeruginosa are prevalent in all three hospitals. The inappropriate and unrestricted use of antibiotics contributes to the development of resistance in hospital-acquired pathogens. It is essential to regularly monitor and document antibiotic resistance to create effective strategies for controlling infections caused by these bacteria.

کلیدواژه ها

Bacteria, Antibiogram, bla-IMP, bla-VIM-۱, bla-VIM-۲

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