Evaluation of Mir-۱۲۹۰ as a Possible Diagnostic Factor in the Serum of Oral Squamous Cell Carcinoma Patients with Qualitative Real-Time Polymerase Chain Reaction

  • سال انتشار: 1403
  • محل انتشار: فصلنامه آسیب شناسی ایران، دوره: 19، شماره: 2
  • کد COI اختصاصی: JR_IJP-19-2_012
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 99
دانلود فایل این مقاله

نویسندگان

Siavash Bagheri Shirvan

Student Research Committee, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

Mehdi Shahabinejad

Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Farnaz Mohajertehran

Department of Oral and Maxillofacial Pathology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

Nazanin Nazari

Student Research Committee, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

چکیده

Background & Objective: This study aimed to determine the incidence of microRNA (miRNA; miR-۱۲۹۰) in the serum of oral squamous cell carcinoma (OSCC) patients compared to a control group using the qualitative real-time polymerase chain reaction (PCR) method.Methods: Blood serum samples were obtained from patients diagnosed with OSCC and confirmed through biopsy. The samples were collected from patients referred to the Mashhad Dental Faculty and Ghaem Hospital. The OSCC group consisted of ۱۷ patients, while the healthy group included ۱۵ individuals. RNA was extracted from the patient samples, and samples with an A۲۶۰/۲۸۰ ratio between ۱.۸ and ۲.۰ (indicating acceptable RNA quality) were immediately converted into complementary DNA (cDNA) using albumin and cDNA reference genes. The SYBR green real-time reverse transcriptase PCR method was used to measure the presence of miR-۱۲۹۰ in the blood samples.Results: A total of ۳۲ patients were examined in this study, including ۱۷ women (۵۳.۱%) and ۱۵ men (۴۶.۹%). The mean age was ۴۶.۷ years in the healthy group and ۵۴.۶ years in the SCC group, indicating a significant difference (P< ۰.۰۵). The expression level of the miR-۱۲۹۰ gene was higher in patients with SCC compared to the healthy group (P=۰.۰۰۰). While the expression level of miR-۱۲۹۰ was higher in grade ۳ and advanced stage than in grades ۲ and ۱ and early stage, the differences were not statistically significant (P=۰.۱۷۳ and P=۰.۵۶۴ for grade and stage, respectively).Conclusion: The expression level of miR-۱۲۹۰ may increase in SCC patients compared to healthy individuals, making it a potential circulating biomarker.  Further investigations for diagnostic utility would be warranted.

کلیدواژه ها

biomarker, miR-۱۲۹۰, Oral Squamous Cell Carcinoma, Quantitative real-time polymerase chain reaction

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.