The Effect of Aerobic Training with Vitamin D on Extrinsic Pathway of Apoptosis and Anti- Apoptotic Indices of Heart Tissue of Rats Exposed to Oxidative Damage Induced by H۲O۲
- سال انتشار: 1396
- محل انتشار: مجله گزارش بهداشت و درمان، دوره: 3، شماره: 1
- کد COI اختصاصی: JR_RHCA-3-1_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 44
نویسندگان
Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Department of Sport Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
چکیده
Introduction: Apoptosis plays an important role in the development of cardiovascular diseases. The aim of this study was to investigate the effect of aerobic training (AT) with Vitamin D (VD) on extrinsic pathway of apoptosis and anti- apoptotic indices of heart tissue of rats exposed to oxidative damage induced by H۲O۲.Methods: In this experimental study, ۶۰ rats were selected and randomly placed into ۱۰ groups of six, including ۱) ۱ mmol / kg H۲O۲, ۲) ۲ mmol / kg H۲O۲, ۳) VD + ۱ mmol / kg H۲O۲, ۴) AT + ۱ mmol / kg H۲O۲, ۵) AT + VD + ۱ mmol / kg H۲O۲, ۶) VD + ۲ mmol / kg H۲O۲, ۷) AT + ۲ mmol / kg H۲O۲, ۸) AT + VD + ۲ mmol / kg H۲O۲, ۹) sham and ۱۰) control. During eight weeks, the rats in groups ۱-۹ were subjected to intraperitoneal injection of H۲O۲ at specific doses three times a week; the rats in groups ۴, ۵, ۷ and ۸ performed aerobic trainings, and the rats in groups ۳, ۵, ۶ and ۸ received ۰.۵ μg / kg of vitamin D۳ intraperitoneally daily. Protein concentrations of Caspase ۸, Fas and FLIP were measured in rat heart tissue. For analysis of the research findings, Kolmogorov- Smirnov, one-way ANOVA, two-way ANOVA along with Bonferroni post hoc tests were used (p≤۰.۰۵). Results: H۲O۲ has a significant effect on the increase of Caspase ۸, Fas and FLIP in the heart tissue of rats (p≤۰.۰۵); eight weeks of aerobic training and Vitamin D intake has a significant effect on decreasing the concentration of Caspase ۸, Fas and increase of FLIP in the heart tissue of rats exposed to oxidative damage induced by ۱ and ۲ m/mol/kg H۲O۲ (p ≤۰.۰۵); aerobic training and Vitamin D intake has significant interactions in the reduction of Caspase ۸, Fas in the heart tissue of rats exposed to oxidative damage induced by ۱ and ۲ m/mol/kg H۲O۲ (p≤۰.۰۵), however, it does not have interactive effect on the increased FLIP of the heart tissue of rats exposed to oxidative damage induced by ۱ and ۲ m/mol/kg H۲O۲ (p≥۰.۰۵). Conclusion: It seems that performing aerobic training activities and simultaneous consumption of vitamin D supplement has interactive effects on reducing some of the extrinsic pathway indices of apoptosis in the heart tissue of H۲O۲-rats exposed to oxidative damage induced by H۲O۲.کلیدواژه ها
Training, Vitamin D, Apoptosis, H۲O۲اطلاعات بیشتر در مورد COI
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