Amir Keshavarzi - Research Center for Behavioral Disorders and Substances Abuse, Hamadan University of MedicalSciences, Hamadan, Iran.
Akram Ranjbar - Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Nejat Kheiripour - Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran.
Ali Ghaleiha - Research Center for Behavioral Disorders and Substances Abuse, Hamadan University of MedicalSciences, Hamadan, Iran.
Alireza Soltaniyan - Department of Epidemiology, Faculty of Health, Hamadan University of Medical Sciences, Hamadan, Iran.
Seyyed Mostafa Hashemi - Research Center for Behavioral Disorders and Substances Abuse, Hamadan University of MedicalSciences, Hamadan, Iran.

Background: Mitochondria are a source of reactive oxygen species (ROS), and several natural compounds are used as antioxidant agents. This study aimed to investigate and compare the effects of fresh grape juice red wine on oxidative stress biomarkers in rat liver mitochondria. Materials and Methods: In this regard, mitochondria were isolated from the liver of ۲۷ male Wistar rats (۲۲۰-۲۵۰ g). The isolated mitochondria were cultured in different doses of red wine and fresh red grape juice for ۲۴, ۴۸, and ۷۲ h. After treatment, total antioxidant capacity, lipid peroxidation, total thiol groups, and catalase activity were determined in the isolated mitochondria of the rat liver. Results: The results confirmed the oxidant/antioxidant effects of red wine and fresh red grape juice at different times. Conclusion: According to the results, the fresh red grape juice showed higher antioxidant properties than red wine in the liver mitochondrial samples.

Antioxidant, Grape juice, Liver mitochondria, Red wine