Supplementation of Freezing Media with Cyclic Adenosine Monophosphate Analog and Isobutylmethylxanthine on Sperm Quality
- سال انتشار: 1399
- محل انتشار: مجله تحقیق در پزشکی مولکولی، دوره: 8، شماره: 4
- کد COI اختصاصی: JR_REMJ-8-4_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 159
نویسندگان
Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran
Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran
Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran
چکیده
Background: This study aimed to explore whether the addition of a cyclic adenosine monophosphate (cAMP) analog and isobutylmethylxanthine (IBMX) in freezing media improved sperm quality and what role cAMP has in this recovery. Materials and methods: ach semen sample was cryopreserved into four groups: fresh semen sample, as a control group, freezing medium + ۲.۵ mM cAMP analog and ۰.۲ mM IBMX, freezing medium + ۱۲.۵ mM cAMP analog and ۰.۲ mM IBMX, and freezing medium + ۲۵ mM cAMP analog and ۰.۲ mM IBMX. Sperm parameters after post-thaw were analyzed according to WHO instruction (۲۰۱۰). Viability, acrosome reaction, and DNA damage levels of the samples were evaluated. Results: Our results indicated that the effective concentrations of ۱۲.۵ and ۲۵ mM cAMP analog and ۰.۲ mM IBMX significantly improved the total motility, progressive motility, and viability of the frozen-thawed (P< ۰.۰۵). However, non-progressive motility and immotile were significantly reduced in the ۱۲.۵ and ۲۵ mM cAMP analogs and ۰.۲ mM IBMX groups after thawing (P< ۰.۰۵). During freezing the spermatozoa, the high concentration of the cAMP analog increased acrosome reaction after thawing in the ۲۵ mM and ۰.۲ mM IBMX treated samples (P< ۰.۰۵). DNA fragmentation in ۲۵ mM cAMP analog and ۰.۲ mM (IBMX) supplementation was significantly lower compared to the other groups (P< ۰.۰۵). Conclusions: Our findings revealed that in vitro cAMP analog and IBMX supplementation in freezing media play an important role in preventing cryodamage by maintaining the sperm functional parameters.کلیدواژه ها
cAMP analog (IBMX), cryopreservation, Asthenozoospermic, sperm qualityاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.