Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-۱۹ Patients with Molecular Detection of Antibiotics Resistance Genes
- سال انتشار: 1402
- محل انتشار: مجله آرشیو رازی، دوره: 78، شماره: 1
- کد COI اختصاصی: JR_ARCHRAZI-78-1_047
- زبان مقاله: انگلیسی
- تعداد مشاهده: 45
نویسندگان
Veterinary Microbiology Department, College of Veterinary Medicine, Al-Qadisiyah University, Al-Qadisiyah, Iraq
Clinical Laboratory Sciences Department, College of Pharmacology, Al-Qadisiyah University, Al-Qadisiyah, Iraq
Clinical Laboratory Sciences Department, College of Pharmacology, Al-Qadisiyah University, Al-Qadisiyah, Iraq
چکیده
Pseudomonas aeruginosa (P. aeruginosa) have a considerable risk to public health in the world, due to its high ability to develop resistance to different classes of antibiotics. It has been discovered as a prevalent coinfection pathogen that causes sickness exacerbation in COVID-۱۹ patients. This study aimed to determine the prevalence of P. aeruginosa from COVID-۱۹ patients in Al Diwaniyah province, Iraq and to identify its genetic resistance pattern. ۷۰ clinical samples were obtained from severe cases of patients (RT-PCR positive for SARS-COV-۲ on a nasopharyngeal swab) who attended Al Diwaniyah Academic Hospital. ۵۰ P. aeruginosa bacterial isolates were detected via microscopic examination, routine cultured and biochemical testing, then validated by the VITEK-۲ compact system. VITEK reported ۳۰ positive results, which later confirmed through molecular detection using ۱۶s RNA specific for detection and a phylogenetic tree.۲۰ isolates had positive PCR findings and ۵ isolates submitted to GenBank with accession numbers OL۳۱۴۵۵۷.۱, OL۳۱۴۵۵۶.۱, OL۳۱۴۵۵۵.۱, OL۳۱۴۵۵۴.۱, OL۳۱۴۵۵۳.۱.For antibiotic resistance genes, the number of the isolates containing blaOXA-۱ and blaCTX-M ۱۸ (۹۰ percent) and ۱۶ (۸۰ percent) respectively. To study its adaptation in a SARS-CoV-۲ infected environment, genomic sequencing investigations were undertaken with phenotypic validation. In conclusion, we demonstrate that multidrug resistant P. aeruginosa play an important role in in vivo colonization in COVID-۱۹ patients and could be one of the causes of death of these patients which indicates the great challenge to clinicians in the facing of this serious disease.کلیدواژه ها
covid-۱۹, Pseudomonas aeruginosa, bla OXA-۱, bla CTX-M, Antibiotic resistance, coinfectionاطلاعات بیشتر در مورد COI
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