Determination of Phenolic Compounds and Antioxidant Activities of ۵۵ Iranian Berberis Genotypes
- سال انتشار: 1402
- محل انتشار: مجله گیاهان دارویی و محصولات فرعی، دوره: 12، شماره: 2
- کد COI اختصاصی: JR_JJMPB-12-2_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 252
نویسندگان
Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
Department of Chemistry, Imam Khomeini International University, Qazvin, Iran
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
Department of Food Safety and Quality Control, Research Institute of Food Science and Technology, Mashhad, Iran
چکیده
The genus Berberis belongs to the Berberidaceae family, with ۱۵ genera and ۶۵۰ species around the world. It has valuable potential in the medicinal and food industries. In this study, the phytochemical diversity of ۵۵ fruits of the Iranian Berberis genotype were investigated. The results of this study could be used in the breeding and determination of superior genotypes in the future. Plant materials were collected from the barberry Collection Garden of Mashhad and also different natural habitats of barberry in various provinces of Iran. The samples were air-dried, finely grounded, and extracted by methanol at room temperature. Then, total phenol and total flavonoid were measured by Folin-Ciocalteu and AlCl۳ assays, respectively. Radicals neutralizing effects of extracts were examined through the ۲,۲-Diphenyl-۱- picrylhydrazyl (DPPH) method. The results showed a significant difference between phenolic content, flavonoid content, and antioxidant properties of various extracts, at a probability level of ۱%. A methanol extract of B. integerrima × crataegina (genotype code ۴-۱) resulted in the highest phenolic content with an average of ۴.۲ mg gallic acid equivalents (GAE) ml-۱ extract, while the lowest content was recorded for B. integerrima) genotype code ۲۳-۴) with an average of ۱.۷ mg gallic acid equivalents (GAE)/ ml extract. The highest flavonoid content was observed with extracts of B. integerrima (genotype code ۴-۴) and B. orthobotrys × crataegina (genotype code ۱۵-۴) with an average of ۶.۳ mg quercetin equivalents (Q)/ ml extract. The least was recorded for B. integerrima (genotype code ۲۳-۴) with an average of ۰.۴ mg quercetin equivalents (Q)/ ml extract. The highest and lowest rates of free radical scavenging DPPH were ۵۹.۰۶% and ۱۲.۳%, respectively. The results showed that barberry has a great diversity in terms of phytochemical characteristics in different genotypes and is a valuable genetic source for breeding research.کلیدواژه ها
Phenolic compound, Folin-Ciocalteu, flavonoid, Antioxidant, Berberisاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.