Bipolaris species associated with rice plant: pathogenicity and genetic diversity of Bipolaris oryzae using rep-PCR in Mazandaran province of Iran
- سال انتشار: 1394
- محل انتشار: Journal of Crop Protection، دوره: 4، شماره: 4
- کد COI اختصاصی: JR_JCP-4-4_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 127
نویسندگان
Department of Plant Protection, Faculty of Agricultural Science and Natural Resources, University of Tehran, Karaj, Iran.
Department of Plant Protection, Faculty of Agricultural Science and Natural Resources, University of Tehran, Karaj, Iran.
Department of Plant Protection, Faculty of Agricultural Science and Natural Resources, University of Tehran, Karaj, Iran.
Department of Plant Protection, Faculty of Agricultural Science and Natural Resources, University of Tehran, Karaj, Iran.
Department of Plant Protection, Faculty of Agricultural Science and Natural Resources, University of Tehran, Karaj, Iran.
چکیده
Ninety one monoconidial Bipolaris isolates were obtained from lesions on different parts of rice in different locations of Mazandaran province during the summer of ۲۰۰۹. Bipolaris species were identified using morphological features such as color and shape of colony and color and size of conidia and conidiophores. The isolates were separated into two species; ۸۵ (۹۳.۴%) isolates belonged to Bipolaris oryzae and the remaining ۶ (۶.۶%) isolates to Bipolaris cynodontis. Therefore B. oryzae is regarded as the major cause of rice brown spot disease in Mazandaran province. In order to analyze genetic diversity among B. oryzae isolates, ۷۱ isolates were subjected to fingerprinting analysis by rep-PCR using BOX and REP primers. In cluster analysis, ۱۵ clonal lineages and ۵۴ haplotypes were identified. The largest clonal lineage contained with ۳۶ haplotypes was the most common lineage. These results also indicate a relatively high level of genetic diversity among B. oryzae isolates. Also, pathogenicity test of a few B. oryzae isolates (۱۲ isolates) was conducted under greenhouse condition and showed that those isolates were pathogenic to rice seedlings of cv. Tarom. All isolates produced some leaf spots ۲۴ h after inoculation.کلیدواژه ها
Bipolaris, Rice, genetic diversity, REP and Box Primers, Pathogenicity test, Mazandaran provinceاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.