Combination Effect of Rotator Cuff Repair with Secretome-hypoxia MSCs Ameliorates TNMD, RUNX۲, and Healing Histology Score in Rotator Cuff Tear Rats
- سال انتشار: 1402
- محل انتشار: مجله استخوان و جراحی عمومی، دوره: 11، شماره: 10
- کد COI اختصاصی: JR_TABO-11-10_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 79
نویسندگان
۱Orthopaedic and Traumatology Division, Surgery Department, Universitas Muhammadiyah Yogyakarta, Indonesia ۲ Doctoral Study Program, Faculty of Medicine, Universitas Padjajaran, Bandung, West Java, Indonesia
۲ Doctoral Study Program, Faculty of Medicine, Universitas Padjajaran, Bandung, West Java, Indonesia ۵ Department of Physiology, Faculty of Medicine, Universitas Padjadjaran, Bandung Indonesia
۲ Doctoral Study Program, Faculty of Medicine, Universitas Padjajaran, Bandung, West Java, Indonesia ۴ Department of Orthopaedics and Traumatology, Faculty of Medicine, Universitas Padjadjaran, Indonesia
۲ Doctoral Study Program, Faculty of Medicine, Universitas Padjajaran, Bandung, West Java, Indonesia ۳ Division of Oncology, Department of Orthopaedic Surgery, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
۶ Stem Cell and Cancer Research, Semarang, Indonesia ۷ Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Semarang, Indonesia ۸ Department of Pathological Anatomy, Faculty of Medicine,
۶ Stem Cell and Cancer Research, Semarang, Indonesia ۹ Graduate student in Biotechnology Department, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia
چکیده
Objectives: In order to treat a rat model of rotator cuff rupture, this work concentrated on the expression of TNMD and RUNX۲, followed by rotator cuff repair and secretome-hMSCs.Methods: A total of thirty ۱۰-weeks-old male Sprague–Dawley rats were separated into five groups randomly, RC on week ۰, lesion treated with a rotator cuff repair and saline (RC + NaCl group, n = ۶) for ۲ and ۸ weeks, and lesion treated with a rotator cuff repair and secretome-hMSCs (RC + secretome-hMSC group, n = ۶) for ۲ and ۸ weeks. The supraspinatus and infraspinatus muscle–tendon units were obtained for histological and biomechanical investigation at ۰, ۲ and ۸ weeks following injury.Results: The findings showed that, in comparison with the RC + NaCl group, secretome-hMSCs significantly improved tendon repair by upregulating TNMD and RUNX۲ expression and histology score.Conclusion: Combining Secretome-hypoxia MSCs with RC healing may help rats with rotator cuff tears. Level of evidence: IVکلیدواژه ها
Mesenchymal stem cells, Rotator cuff tear, RUNX۲, Secretome, TN MDاطلاعات بیشتر در مورد COI
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