Dendrosomal nanocurcumin prevents EBV-associated cell transformation by targeting the lytic cycle genes of the Epstein-Barr virus in the generation of lymphoblastoid cell line
- سال انتشار: 1402
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 26، شماره: 10
- کد COI اختصاصی: JR_IJBMS-26-10_013
- زبان مقاله: انگلیسی
- تعداد مشاهده: 224
نویسندگان
Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran
Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
چکیده
Objective(s): Targeting the lytic cycle of the Epstein-Barr virus (EBV) has been considered a new treatment strategy for malignancies caused by this virus. This study aimed to investigate the effect of Dendrosomal NanoCurcumin (DNC) to prevent cell transformation and inhibit the expression of viral lytic gene expression in the generation of lymphoblastoid cell line (LCL).Materials and Methods: Cell viability of LCLs and PBMCs was performed by MTT assay, and flow cytometry (Annexin/PI) was used for evaluation of apoptosis. CD markers on the surface of generated LCL (CD۱۹) cells were examined for cell validation. The effect of DNC on transformation was evaluated by examining cell morphology and determining the expression level of lytic genes BZLF۱, Zta, BHRF۱, and BRLF۱ of EBV using Real-time PCR. Student’s t-test was used for statistical analysis.Results: The MTT assay showed that DNC can inhibit the proliferation of LCL in a dose-dependent manner. The ۵۰% cytotoxic concentration (CC۵۰) of DNC and curcumin for LCL was determined ۳۸.۸ µg/ml and ۷۵ µg/ml, respectively after ۷۲ hr. Also, Real-time PCR data analysis showed that DNC in ۳۰ µg/ml concentration significantly inhibited cell transformation in the LCL and significantly reduced viral lytic genes such as BZLF۱, Zta, BHRF۱, and BRLF۱expression compared to control.Conclusion: Overall, these findings show that DNC reduces the expression of the viral lytic cycle genes and also the induction of cell apoptosis and finally prevents the generation of LCL.کلیدواژه ها
Cell transformation, Curcumin, Epstein-Barr virus, Lymphoma, Viral genesاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.