The Effects of Acrylamide on Fertilization Potentialand Sperm Parameters in Mice
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
- کد COI اختصاصی: RROYAN23_090
- زبان مقاله: انگلیسی
- تعداد مشاهده: 124
نویسندگان
Department of Basic Sciences, Faculty of Veterinary Medicine, UrmiaUniversity, Urmia, Iran
Department of Basic Sciences, Faculty of Veterinary Medicine, UrmiaUniversity, Urmia, Iran
Department of Basic Sciences, Faculty of Veterinary Medicine, UrmiaUniversity, Urmia, Iran
Department of Basic Sciences, Faculty of Veterinary Medicine, UrmiaUniversity, Urmia, Iran
چکیده
Background: Today, with changing of lifes tyle, the tendency touse packaged food, s treet food and fas t food has been increased.Many of these foods contain Acrylamide. One of the mos t importantside effects of Acrylamide is toxicity in the reproductivesys tem. In this s tudy, the long-term effects of Acrylamide onin vitro Fertilization (IVF) and sperm parameters were investigated.Materials and Methods: Sixteen male mice (age: ۶-۸ weeks)were divided into two groups. The control group has receivednormal saline (۰.۲ ml/day) and treated group received Acrylamide(۵۰ mg/kg, ۰.۲ ml/day) orally for ۴۵ days. At the end of theterm, sperms were extracted from the tail of the epididymis andfertilization process was performed in HTF + ۴mgBSA mediumand embryonic growth s tages were s tudied during ۱۲۰ hours ofincubation. Also, sperm count, motility, viability, sperm chromatinquality, and DNA integrity were evaluated. Two proportionmethod by Minitab software and T-tes t by SPSS softwarewere used for s tatis tical analysis (P< ۰.۰۵).Results: There was a significant decrease in the percentage offertilization, ۲cell-embryos and blas tocys ts resulting from invitro fertilization and a significant increase in the number of arrested embryos in treated group by Acrylamide compared withthe control group (P< ۰.۰۵). Also in treated group by Acrylamide,sperm count, motility , and viability of sperms, the number of sperms with DNA damage, abnormal morphology, andwith immature nucleus showed a significant increase comparedwith the control group (P< ۰.۰۵) .Conclusion: The s tudy showed long-term oral adminis trationof Acrylamide has negative effects on fertility potential andsperm parameters.کلیدواژه ها
Acrylamide, Fertilization Potential, Mice, NucleusMaturation, Sperm Qualityاطلاعات بیشتر در مورد COI
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