The Effects of Acrylamide on Fertilization Potentialand Sperm Parameters in Mice

Background: Today, with changing of lifes tyle, the tendency touse packaged food, s treet food and fas t food has been increased.Many of these foods contain Acrylamide. One of the mos t importantside effects of Acrylamide is toxicity in the reproductivesys tem. In this s tudy, the long-term effects of Acrylamide onin vitro Fertilization (IVF) and sperm parameters were investigated.Materials and Methods: Sixteen male mice (age: ۶-۸ weeks)were divided into two groups. The control group has receivednormal saline (۰.۲ ml/day) and treated group received Acrylamide(۵۰ mg/kg, ۰.۲ ml/day) orally for ۴۵ days. At the end of theterm, sperms were extracted from the tail of the epididymis andfertilization process was performed in HTF + ۴mgBSA mediumand embryonic growth s tages were s tudied during ۱۲۰ hours ofincubation. Also, sperm count, motility, viability, sperm chromatinquality, and DNA integrity were evaluated. Two proportionmethod by Minitab software and T-tes t by SPSS softwarewere used for s tatis tical analysis (P<۰.۰۵).Results: There was a significant decrease in the percentage offertilization, ۲cell-embryos and blas tocys ts resulting from invitro fertilization and a significant increase in the number of arrested embryos in treated group by Acrylamide compared withthe control group (P<۰.۰۵). Also in treated group by Acrylamide,sperm count, motility , and viability of sperms, the number of sperms with DNA damage, abnormal morphology, andwith immature nucleus showed a significant increase comparedwith the control group (P<۰.۰۵) .Conclusion: The s tudy showed long-term oral adminis trationof Acrylamide has negative effects on fertility potential andsperm parameters.

کلیدواژه ها:

Acrylamide ، Fertilization Potential ، Mice ، NucleusMaturation ، Sperm Quality