Morphological and phylogenetic investigation on selected Fusarium species belong to Gibberella fujikuroi species complex in Iran
- سال انتشار: 1392
- محل انتشار: دوفصلنامه رستنیها، دوره: 14، شماره: 2
- کد COI اختصاصی: JR_ROST-14-2_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 192
نویسندگان
MSc graduated, Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Prof., Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran (jnikkhah@ut.ac.ir)
Assistant Prof., Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
PhD Student, Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده
The Gibberella fujikuroi species complex with Fusarium anamorphs is the causal of diverse diseases of many important plants. Many of the known species in this species complex produce a wide range of secondary metabolites. In this study, morphological characteristics of ۴۶ isolates from rice, maize, sugarcane and onion and phylogenetic relationships of ۲۲ isolates on the basis of ITS۱-۵.۸S-ITS۲ region and EF-۱α gene sequences were investigated. According to the morphological characteristics of ۴۶ isolates, five species including F. fujikuroi, F. proliferatum, F. sacchari, F. thapsinum, and F. verticillioides were identified. Moreover, earlier morphologically two isolates obtained from sugarcane were very similar to F. verticillioides but, using EF-۱α gene sequence were identified as F. andiyazi. On the basis of EF-۱α sequence, six clades were identified and differentiated all of six species (F. andiyazi, F. fujikuroi, F. proliferatum, F. sacchari, F. thapsinum and F. verticillioides) from one another. Moreover, sequencing of ITS۱-۵.۸S-ITS۲ region identified five clades and differentiated F. proliferatum and F. verticillioides from each other but this region could not differentiate F. proliferatum and F. fujikuroi or F. andiyazi and F. verticillioides from each other. Generally, EF-۱α gene was appropriate for differentiation of Gibberella fujikuroi species complex.کلیدواژه ها
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.