Anti-nociceptive Activity of Quebracho tannin Extract on Pain Induced by Formalin and Writhing Tests in Mice

  • سال انتشار: 1400
  • محل انتشار: مجله بیوشیمی پزشکی، دوره: 9، شماره: 2
  • کد COI اختصاصی: JR_MEBIO-9-2_003
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 239
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نویسندگان

Fatemeh Zare

Graduate Student, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

Shahin Hassanpour

Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

Ahmad Asghari

Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

Alireza Jahandideh

Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

چکیده

Background: Based on positive role of the tannins for pain relief, there is no report for possible antinociceptive activity of the Quebracho tannin. Objectives: This study aimed to determine the anti-nociceptive activity of the Quebracho tannin extract (QTE) on pain in mice. Materials and Methods: For this purpose, ۳۴۰ mice were used for formalin and writhing tests each including ۴ experiments with ۴ sub-groups. In experiment ۱, mice were injected with saline, QTE (۱۰۰ mg/kg), QTE (۲۰۰ mg/kg), QTE (۴۰۰ mg/kg), and morphine (۵ mg/kg). In the second experiment, injections included saline, QTE (۴۰۰ mg/kg), naloxone (۲ mg/kg), and QTE + naloxone. Experiments ۳ and ۴ were similar to experiment ۲, except that mice injected were with NG-nitro arginine methyl ester (L-NAME, ۱۰ mg/kg) and cyproheptadine (۴ mg/kg) instead of naloxone. Then, formalin (۱%) was injected, and time spent for licking the injected paw was recorded until ۳۰ minutes following injection in the first and second phases. Finally, injections in ۴ experiment groups were the same, and animals were intraperitoneally injected with acetic acid, and contractions were recorded in the writhing test category. Results: According to the results, QTE (۱۰۰, ۲۰۰, and ۴۰۰ mg/kg) decreased pain in the injected paw (P = ۰.۰۰۱) and inhibited the pain response by ۵۹.۳۷% (P = ۰.۰۰۱). Moreover, the injection of naloxone + QTE significantly decreased pain in the injected paw (P = ۰.۰۲۱). Eventually, the injection of the L-NAME + QTE significantly reduced the anti-nociception effect of the QTE on the formalin test (P = ۰.۰۳۱) and writhing contractions (۵۵.۷۵%, P = ۰.۰۳۳). Conclusion: These findings suggested anti-nociceptive properties of the QTE mediated by opioidergic and nitrergic systems.

کلیدواژه ها

Quebracho tannin, Pain, Formalin test, Mice

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