Effect of isolated polysaccharide from Rosa canina on the methylation of insulin and Pax۴ in diabetic rats
- سال انتشار: 1400
- محل انتشار: سومین کنگره بین المللی و چهارمین همایش ملی زیست فناوری گیاهان دارویی و قارچهای کوهی (مجازی)
- کد COI اختصاصی: BMPM04_041
- زبان مقاله: انگلیسی
- تعداد مشاهده: 381
نویسندگان
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
چکیده
Introduction: Since DNA methylation plays a causal role in β-cell remodeling and development, thus modulation of this epigenetic mechanism is essential in treatment of diabetes. Therefore, this provoked us to examine the effect of a known anti-diabetic agent, an isolated polysaccharide, on the methylation pattern of Ins-۱ and Pax-۴ in diabetic rats. Materials: Here, a polysaccharide fraction was isolated from Rosa canina and analyzed using NMR, FTIR and MS/MS techniques. Diabetes was established by using intraperitoneal injection of STZ in male Wistar rats. After treatment, pancreas was removed and DNA was extracted and bisulfite treated by a DNA methylation kit. PCR and real-time PCR were used to determine the levels of methylated and/or unmethylated Ins-۱ and Pax-۴ genes.Results: The levels of blood glucose and weight body were normalized in diabetic rats exposed to isolated polysaccharide. The level of unmethylated Ins-۱ was upregulated in diabetic rats which is downregulated in metformin and polysaccharide-treated ones. In diabetic rats, the content of methylated Pax-۴ was increased while it was decreased in polysaccharide-treated group. Interestingly, the methylation pattern of Pax-۴ in metformin group was the same as diabetic ones.Conclusion: Data clearly indicated that polysaccharide can reduce the level of blood glucose by modulating the methylation pattern of Pax-۴ and Ins-۱. This study sheds light on the importance of DNA methylation modulation as a promising therapeutic strategy in diabetes.کلیدواژه ها
Diabetes, DNA methylation, Polysaccharide, Rosa caninaمقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.