AKT family and miRNAs expression in IL-۲-induced CD۴+T cells
- سال انتشار: 1393
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 17، شماره: 11
- کد COI اختصاصی: JR_IJBMS-17-11_010
- زبان مقاله: انگلیسی
- تعداد مشاهده: 217
نویسندگان
Department of Genetics, College of Science, Rasht Branch, Islamic Azad University, Rasht, Iran
Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Objective(s): Study of non-coding RNAs is considerable to elucidate principal biological questions or design new therapeutic strategies. miRNAs are a group of non-coding RNAs that their functions in PI۳K/AKT signaling and apoptosis pathways after T cell activation is not entirely clear. Herein, miRNAs expression and their putative targets in the mentioned pathways were studied in the activated CD۴+T cells. Materials and Methods: Herein, proliferation rate and IL-۲ secretion were measured in treated and untreated cells by IL-۲. Putative targets of up-regulated miRNAs were predicted by bioinformatics approaches in the apoptotic and PI۳K/AKT signaling pathways. Then the expression of two putative targets was evaluated by quantitative RT-PCR. Results: Proliferation rate of treated cells by IL-۲ increased in a dose- and time- dependent manner. Naive and activated CD۴+T cells induced by different dose of IL-۲ secreted abundant amounts of IL-۲. Also, in IL-۲ un-induced cells (IL-۲ depleted cells) after ۳ days, decrease of proliferation has been shown. In silico analysis predicted putative targets of up-regulated miRNAs such as AKT۱, AKT۳ and apoptotic genes in the activated cells induced or un-induced by IL-۲. Decrease of AKT۳ was shown by Q-RT-PCR as a potential target of miRNAs overexpressed in IL-۲ depleted cells. But there was no significant difference in AKT۱ expression in two cell groups. Conclusion: Our analysis suggests that decrease of AKT۳ was likely controlled via up-regulation of specific miRNAs in IL-۲ depleted cells. Also it seems that miRNAs play role in induction of different apoptosis pathways in IL-۲ induced and un-induced cells.کلیدواژه ها
AKT۳, IL-۲ induction, In silico analysis, miRNAsاطلاعات بیشتر در مورد COI
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