Survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold

  • سال انتشار: 1395
  • محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 19، شماره: 12
  • کد COI اختصاصی: JR_IJBMS-19-12_002
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 390
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نویسندگان

Sajad Sahab Negah

Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

Hadi Aligholi

Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

Zabihollah Khaksar

Histology and Embryology group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

Hadi Kazemi

Pediatric Department, Medical Faculty, Shahed University, Tehran, Iran

Sayed Mostafa Modarres Mousavi

Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran

Maryam Safahani

Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran

Parastoo Barati Dowom

Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran

Ali Gorji

Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran

چکیده

Objective(s): In order to grow cells in a three-dimensional (۳D) microenvironment, self-assembling peptides, such as PuraMatrix, have emerged with potential to mimic the extracellular matrix. The aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma stem-like cells (hMgSCs). Materials and Methods: The efficacy of a novel method for placing hMgSCs in PuraMatrix (the injection approach) was compared to the encapsulation and surface plating methods. In addition, we designed a new method for measurement of migration distance in ۳D cultivation of hMgSCs in PuraMatrix. Results: Our results revealed that hMgSCs have the ability to form spheres in stem cell culture condition. These meningioma cells expressed GFAP, CD۱۳۳, vimentin, and nestin. Using the injection method, a higher proliferation rate of the hMgSCs was observed after seven days of culture. Furthermore, the novel migration assay was able to measure the migration of a single cell alone in ۳D environment. Conclusion: The results indicate the injection method as an efficient technique for culturing hMgSCs in PuraMatrix. Furthermore, the novel migration assay enables us to evaluate the migration of hMgSCs.

کلیدواژه ها

brain tumor, Cell culture, Cell migration, RADA۱۶-I, Stem cells

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