Quantitative evaluation of human sperm viability using MTT assay: A laboratory study
- سال انتشار: 1399
- محل انتشار: International Journal of Reproductive BioMedicine، دوره: 18، شماره: 11
- کد COI اختصاصی: JR_IJRM-18-11_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 394
نویسندگان
Department of Biology, Faculty of Science, Arak University, Arak, Iran.
Department of Biology, Faculty of Science, Arak University, Arak, Iran.
Department of Biology, Faculty of Science, Arak University, Arak, Iran.
چکیده
Background: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability. Objective: This study was performed to assess human sperm viability using MTT assay. Materials and Methods: In this laboratory study, human-ejaculated semen samples (n = 56 from different donors) were used. The sperm viability was determined using quantitative MTT assay and the sperm motility was assessed according to World Health Organization guidelines. Sperm viability and the correlation between sperm viability and motility were analyzed. Results: Data revealed a marked positive correlation between MTT reduction rate and the percentage of viable spermatozoa. The Pearsonchr('39')s correlation coefficients also showed a significant correlation between sperm viability and motility. Conclusion: MTT assay which is based on mitochondrial functionality is a reliable method for evaluating human sperm viability and could be used as a diagnostic test for predicting sperm fertilization ability in clinical settings.کلیدواژه ها
Human sperm, Viability, MTT assay, اسپرم انسان, قابلیت حیات, سنجش MTT.اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.