Assessment of Genetic Diversity by Application of Inter Simple Sequence Repeat (ISSR) Primers on Iranian Harmal (Peganum harmala L.) Germplasm as an Important Medicinal Plant

  • سال انتشار: 1395
  • محل انتشار: فصلنامه گزارش های زیست فناوری کاربردی، دوره: 3، شماره: 3
  • کد COI اختصاصی: JR_JABR-3-3_002
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 404
دانلود فایل این مقاله

نویسندگان

Alireza Zebarjadi

Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University, Kermanshah, Iran|Biotechnology for Drought Tolerance Research Department, Razi University, Kermanshah, Iran

Hossein Rostami Ahmadvandi

Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University, Kermanshah, Iran

Danial Kahrizi

Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University, Kermanshah, Iran|Biotechnology for Drought Tolerance Research Department, Razi University, Kermanshah, Iran

Kianoosh Cheghamirza

Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University, Kermanshah, Iran|Biotechnology for Drought Tolerance Research Department, Razi University, Kermanshah, Iran

چکیده

Syrian Rue or Harmal (Peganum harmala L.), belonged to the family of Peganaceae, grows in semi-arid climates such as the Middle East and North Africa. Traditionally, this plant, especially the seeds, has been recognized for its several medicinal uses. In this stydy, genetic diversity between 21 Harmal accessions, collected from different regions of Iran were evaluated by Inter Simple Sequence Repeat (ISSR) marker using 14 spesific primers. All primers successfully amplified polymorphism regions, as among 115 regions, 68 polymorphic regions (59.13%) were amplified. Accordingly, highest and lowest similarities among ecotypes were measured as 94% and 55%, respectively. The results based on cluster analysis also categorized all accessions into three groups that did not completely match to their geographic pattern place of collection. Result of principle coordinate analysis of samples also showed distribution pattern similar to cluster analysis.

کلیدواژه ها

Peganum harmala, genetic diversity, ISSR, Cluster analysis

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.