Anticonvulsive effects of endocannabinoids; an investigation to determine the role of regulatory components of endocannabinoid metabolism in the Pentylenetetrazol induced tonic- clonic seizures
محل انتشار: پانزدهمین کنگره بین المللی صرع ایران
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 468
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شناسه ملی سند علمی:
EPILEPSEMED15_147
تاریخ نمایه سازی: 29 اردیبهشت 1398
چکیده مقاله:
2-Arachidonoylglycerol (2-AG) and anandamide are two major endocannabinoids produced, released and eliminated by metabolic pathways. Anticonvulsive effect of 2-AG and CB1 receptor is well-established. Herein, we designed to investigate the anticonvulsive influence of key components of the 2-AG and anandamide metabolism. Tonic-clonic seizures were induced by an injection of Pentylenetetrazol (80 mg/kg, i.p.) in adult maleWistar rats. Delay and duration for the seizure stages were considered for analysis. Monoacylglycerol lipase blocker (JJKK048; 1 mg/kg) or alpha/beta hydroxylase domain 6 blocker (WWL70; 5 mg/kg) were administrated alone or with 2-AG to evaluate the anticonvulsive potential of these enzymes. To determine the CB1 receptor involvement, its blocker (MJ15; 3 mg/kg) was dministrated associated with JJKK048 or WWL70.To assess anandamide anticonvulsive effect, anandamide membrane transporter blocker (LY21813240;2.5 mg/kg)was used alone or associated with MJ15. Also, fatty acid amide hydrolase blocker (URB597; 1 mg/kg; to prevent intracellular anandamide hydrolysis) were used alone or with AMG21629 (transient receptor potential vanilloid; TRPV1 antagonist; 3 mg/kg). All compounds were dissolved inDMSO and injected i.p.,before the Pentylenetetrazol. Both JJKK048 and WWL70 revealed anticonvulsive effect. Anticonvulsive effect of JJKK048 but not WWL70 was CB1 receptor dependent. LY2183240 showed CB1 receptor dependent anticonvulsive effect. However, URB597 revealed a TRPV1 dependent proconvulsive effect. It seems extracellular accumulation of 2-AG or anandamide has anticonvulsive effect through the CB1 receptor, while intracellular anandamide accumulation is proconvulsive through TRPV1.
کلیدواژه ها:
2-Arachidonoylglycerol . ABHD6 . Anandamide . CB1 receptor .MAGL . Rat . TRPV1
نویسندگان
Parisa Zareie
Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
Homeira Moradi-Chameh
Division of Fundamental Neurobiology, Krembil Research Institute, Toronto, ON, Canada
Mehdi Sadegh
Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
Mohammad Reza Palizvan
Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran