Evaluation of the microbiological quality of the environment in a laboratory of microbiology candidate to the accreditation

The microbiological quality of the environment of two laboratories of microbiological quality control of the agro alimentary products, the first candidate to the accreditation and the second not candidate to the accreditation was evaluated. The samples of air were taken by aspiring the air, through physiological water of a biocollector, rooms of laboratories to a flow of ۲L/min. The test sample selection of surfaces was carried out by cleaning. In these samples, the totals aerobic Germs mesophilic, Yeasts and Moulds, Totals coliforms, Staphylococcus aureus were sought and counted. On the whole, ۱۲۰ samples were analyzed including ۴۵ samples of surfaces and ۱۵ samples of air of each laboratory, for three months. The results show that in the Laboratory in Process of Accreditation the concentration of the Mesophilic Aerobic Bacteria total and the Yeasts Moulds respectively ۴۲ UFC / ۲۵cm۲ and ۳۷ UFC / ۲۵cm۲ and with ۸۴% of samples in conformity. In the air of the same laboratory, the concentrations were of ۱۰UFC/۱۰۰۰cm ۳ of esophilic Aerobic Bacteria and ۱۰ UFC/۱۰۰۰cm ۳ of Yeasts Moulds with ۱۰۰% of conformity. On the other hand, the Laboratory Not in Process of Accreditation, concentration of the Mesophilic Aerobic Bacteria and the Yeasts and Moulds respectively ۱۲۸ UFC / ۲۵cm۲ and ۴۵ UFC / ۲۵cm۲ and with ۶۰% of samples in conformity. In the air of the same laboratory, the concentrations were of ۱UFC/۱۰۰۰cm۳ of Mesophilic Aerobic Bacteria and ۴۲ UFC/۱۰۰۰cm ۳ of Yeasts Moulds with ۶۰% of conformity. The standards of microbiological quality were not still reached in the two laboratories; however those of the candidate to the accreditation were twice closer to the standards of the accreditation than the no laboratory in Process of accreditation.

کلیدواژه ها:

Evaluation ، Microbiological quality ، Environment ، Laboratory of microbiology ، Accreditation