Lack of expression of drought microRNAs in the leaves of Narcissus pseudonarcissus L. under drought stress

Advances in the world are causing changes in the living conditions of living organisms such as plants, and therefore with different methods, including molecular methods, must cope with environmental stressful conditions, such as drought stress. One mechanism is the regulation of gene expression after transcription by microRNAs(miRNAs). MiRNAs are non-coding nucleotides (length ۲۰-۲۲). Previous studies have shown that the expression of miRNAs can change in response to drought stress. Due to its many decorative, medicinal-health uses, the daffodil plant is expanding and becoming profitable not only in the domestic markets but also in its exports. Due to the vastness of arid and semi-arid climates in Iran, studies that can be effective in strengthening this plant with dehydration conditions in the future are useful and necessary. For this purpose, Narcissus bulbs were planted under different irrigation conditions (from once a week to once every two months). From the leaves of plants after two months, to extract the target gene of drought-related miRNAs, which according to the list in the NCBI gene bank, were micro RNA ۱۵۹, with the target gene MYB and micro RNA ۱۷۲, with the target gene AP۲, by method qPCR was used. The two genes AP۲ and MYB belong to transcription factors. The experimental results showed that despite the increase in expression of drought-related genes under drought stress, miRNAs did not show any change in expression, so it is possible that the lack of expression of miRNAs is somehow related to the increase in drought-related genes. Due to the fact that the expression of drought genes and related miRNAs has been evaluated for the first time in Narcissus pseudonarcissus therefore this study can provide a good basis for further study in this context.

کلیدواژه ها:

MYB ، AP۲ ، microRNA ۱۵۹ ، microRNA ۱۷۲