Immediate administration of hTERT-MSCs-IDO۱-EVs reduces hypoalbuminemia after spinal cord injury

Spinal cord injury (SCI) presents challenging and unpredictable neurological recovery. During inflammatory conditions, the amount of serum albumin and nutrition consumption decreases. Currently, it is proposed to measure serum albumin and glucose content in human or animal subjects to predict the recovery rate and the efficiency of treatments following SCI. In this study, the effect of extra-cellular vesicles (EVs) from immortalized human adipose tissue-derived mesenchymal stem cells (hTERT-MSCs) equipped with the ectopic expression of the human indoleamine ۲,۳-dioxygenase-۱ (IDO۱) gene on serum albumin and glucose levels was investigated. After pre-clearing steps of ۷۲-hr conditioned media, small EVs (sEVs) were isolated based on the ultra-filtration method. They were encapsulated with a chitosan-based hydrogel. Five experimental groups (female rats, N = ۳۰, ~ ۲۳۰ g) were considered, including SCI, sham, hydrogel, control green fluorescent protein (GFP)-EVs and IDO۱-EVs. The ۶۰.۰۰ µL of hydrogel or hydrogels containing ۱۰۰ µg sEVs from GFP or IDO۱-EVs were locally injected immediately after SCI (laminectomy of the T۱۰ vertebra and clip compression). After ۸ weeks, non-fasting serum glucose and albumin levels were measured. The results indicated that the level of serum albumin in the animals received IDO۱-EVs (۳.۵۲ ± ۰.۰۴) was increased in comparison with the SCI group (۳.۰۰ ± ۰.۹۴). Also, these animals indicated higher glucose levels in their serum (۲۵۰.۱۷ ± ۶۹.۶۱) in comparison with SCI ones (۲۱۴ ± ۴۵.۳۴). Although these changes were not statistically significant, they could be considered as evidence for the beneficial effects of IDO۱-EVs administration in the context of SCI to reduce hypoalbuminemia and improve energy consumption. More detailed experiments are required to confirm these results.

کلیدواژه ها:

Extra-cellular vesicles ، Glucose ، Hypoalbunemia ، Indoleamine ۲ ، ۳-dioxygenase-۱ ، Spinal Cord Injury