Effect of Apigenin on FOXO, SOD۲ geneexpression in colorectal cancer cell

Introduction: Colorectal cancer (CRC) is one of the mostprevalent and lethal cancers worldwide. Apigenin is a naturalflavonoid that exhibits potential antitumor effects on varioushuman cancer cell lines. Since ROS plays a crucial role in cancerand the effect of apigenin on ROS has been demonstrated,we selected Foxo and SOD۲ genes as our targets. Foxo is a transcriptionfactor that regulates the expression of SOD۲, which isa key cellular antioxidant that converts two molecules of superoxideanion into hydrogen peroxide and oxygen. By activatingSOD۲, Foxo reduces the harmful superoxide anions.Materials and Methods: The effect of apigenin on Foxo andSOD۲ expression in colorectal cancer cells was investigated.For this purpose, SW۴۸۰ colorectal cancer cells were treatedwith different concentrations of apigenin and a MTT assaywas performed to determine the cytotoxic effect of apigenin onSW۴۸۰ cell viability and calculate the IC۵۰. Moreover, scratchassay was used to examine the effect of apigenin on cell migrationand metastasis. Then, using the obtained IC۵۰, RNAextraction was performed and cDNA synthesis was carried out.Finally, gene expression was measured using real-time PCRwith ACTB gene as a control.Results: Apigenin had a cytotoxic effect on SW۴۸۰ cells andthe cells treated in concentrations of ۰, ۶.۲۵, ۱۲.۵, ۲۵, ۵۰, ۱۰۰,۱۵۰, ۳۰۰ μM in ۴۸ hours and with high concentration (۳۰۰μM) of apigenin had lower viability. The IC۵۰ of apigeninfor SW۴۸۰ cells was ۸۴ μM as measured by MTT assay. Thechanges in gene expression were detected by real-time PCR.Conclusion: Apigenin has an anticancer effect and it is possiblethat apigenin modulates ROS levels through Foxo andSOD۲ genes. Apigenin has low toxicity and may be a promisingcandidate for CRC treatment.

کلیدواژه ها:

Apigenin ، ROS ، CRC ، cancer