Effect of Surface Microbiome and Osmo-Conditioning on Restoration of Storage-Induced Losses of Seed Viability in Muskmelon (Cucumis melo L.)

Seed priming can restore age-induced loss of seed viability or longevity; however, these invigoration responses may vary with priming agents and seed aging duration. The present study investigated the effect of two potassium salts (K-salts) individually and in combination (۱:۱) on different lots of muskmelon genotype, MS-۱ seeds stored for four consecutive years, from ۲۰۱۳ to ۲۰۱۶ under ambient conditions. The combination of K-salts significantly enhanced the percent germination of seeds stored for two years (from ۲۰۱۵ to ۲۰۱۶). Further, it also improved root morpho-traits of ۱۰-day-old seedlings. The storage duration had significant effect on the seed surface bacterial and fungal populations. A significantly higher cfu mL-۱ microbial counts were recorded for ۲۰۱۳ harvested seeds on three different agar-based media compared to ۲۰۱۴ to ۲۰۱۶ stored seeds. Further, the Scanning EM and FT-IR study revealed the surface microbiological status and functional groups variations, respectively. Thus, aging-related seed coat microflora is responsible for deterioration of the seed coat. Osmo-conditioning cannot restore viability of seeds stored under ambient conditions for more than two years.

کلیدواژه ها:

Electron microscopy ، Melon ، Osmolyte ، Seed priming ، Storage time ، Surface microbes.