Mathematical Modeling of Prediction of AntibacterialProperties of the Combination Honey with Alcoholic Extracts of BlackCardamom, and Zataria multiflora

Background and Aim : Mathematical modeling is widely used by scientists in biological fields.Using modeling can help to choose the best method of predicting and controlling a biologicalmechanism. Mathematical models can estimate quantities that cannot be directly accessed. On theother hand, during the last forty years, many efforts have been made to find new antibiotics thatare clinically useful. It is felt to use natural substances and plant extracts to control and treatinfections. Since honey and Zataria multiflora and Black Cardamom plants contain effectiveantimicrobial substances such as thymol, carvacrol, parsimol, resorcinol and hydrogen peroxide,They have the ability to kill or inhibit the growth of some pathogenic microorganisms, Thereforeaim of this study is to predict the mathematical model of antimicrobial effects of honey, Zatariamultiflora and black cardamom on Escherichia coli bacteria.Methods : The model was created for this combination, and then this model was implemented inMATLAB software to obtain the most optimal parameters. Then, the antibacterial properties ofthe above compounds were extracted and evaluated in the laboratory environment with theobtained parameters.Results : Our mathematical model measured the cultivation time on the desired solutions showedthat the concentration of the solution is related to the suppression of bacterial growth., Thecombination of honey ۳۰%, Zataria multiflora ۳۵%, and Black Cardamom ۳۵% had an inhibitoryand lethal effect on Escherichia coli ATCC۲۵۹۲۲ bacterium, MIC/MBC=۱۰۰۰ μg/ml.Conclusion : The obtained results were in coincide with the evaluation of the model, which finallyshowed the antimicrobial properties of the above compounds correctly. These compounds haveinhibitory effects on Escherichia coli, Therefore, the use of mathematical models to predict theeffects of organisms and optimal controls on laboratory methods seems to be a useful tool.

کلیدواژه ها:

Mathematical prediction ، Modeling ، Antibacterial ، Honey ، Black cardamom ، Zatariamultiflora.