In silico transcriptome analysis of drought and salt involved responsiveness genes in Brassica napus

Canola (Brassica sp.) as the most important oily seed product in the world is affected largely by salinity anddrought stresses due to planting in arid and semi-arid regions. So, studying about impressive genes insalt/drought stresses in canola would be helpful to improve tolerant species. In this study genes that involvedin salt and drought stresses in Brassica napus were investigated from integrating gene expression omnibus(GEO) and sequence read archive (SRA) databases in different time line since ۲۰۲۱. Then results wereanalyzed by web based software GEO۲R and GALAXY for detect differentially expressed genes (DEGs).DEGs in short, mid and long-term of salinity and drought stresses were obtained from extensive metaanalysis(RRA method). Subsequently ontology of acquired robustDEGs were done by blast۲go. Byconstructing PPI network with Cytoscape software, hub genes in each time line were identified. Among all۳۴ A. thaliana hub genes, HAI۲ and DREB۱B were selected to validate with real time qPCR in tolerant(Okapi) and sensitive (RGS) cultivars of canola. The hub known and novel genes that identified through ourmeta-analysis, provide insight to understanding the molecular response to salinity/drought stresses andengineering abiotic stress tolerance in canola.

کلیدواژه ها:

Brassica napus ، salinity ، drought ، meta analysis ، candidate genes