INSIGHT TO CYCLIND1 AND CDK4 ACTIVITY FOLLOWING REPERFUSION IN INDUCED TESTICULAR TORSION IN RATS

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 329

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شناسه ملی سند علمی:

MHMIMED04_016

تاریخ نمایه سازی: 13 اسفند 1398

چکیده مقاله:

Background and Aim : One of the most important emergency surgical conditions is known as testicular torsion (TT), which disrupts the physiological condition of testicular tissue and ends up spermatogenesis arrest. The testicular reperfusion (TR) surgery has been recognized as the only therapeutic approach to improve the TT-induced damages. The cylinD1 and Cdk4 are known as the cell cycle modulator proteins, which positively control the spermatogonial stem cells (SSCs) transition from G1 to S phase during mitosis. Therefore, the current study was performed to demonestrate the testicular tissue survival as well as cylinD1 and Cdk4 protein levels in SSCs following TT and TR in a time dependent manner.Methods : Thirty male healthy Wistar rats were randomized into two control (N=6) and experimental groups (N=24). In the experimental group animals underwent unilateral right testis TT for 2 hours (N=24 rats in each group). Following 2 hours of TT, the testicular tissues were dissected out from 6 rats and the other 18 rats were undergone TR for 1, 2 and 4 hours. Finally, the cylinD1 and Cdk4 proteins levels were analyzed using immunohistochemistry staining.Results : The TT group animals showed cyclinD1 and Cdk4 proteins reduction versus control group animals. The TR-induced animals exhibited enhanced cylinD1 and Cdk4 protein levels in a time dependent manner. In 4 hours post reperfusion group, cylinD1 protein level was increased significantly compared to TT-induced group, while there was no significant differences in 1 and 2 hours post reperfusion compared to TT-induced group (P<0.05).Conclusion : Considering enhanced cylinD1 and Cdk4 proteins levels in 4-hour TR-induced animals, it could be concluded that, at least a 4- hour post reperfusion period is required to reduce the TT-induced cell cycle arrest and re-initiate the machinery.

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نویسندگان

Shokuh Mardani

Departmemt of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Rahim Mohammadi

Departmemt of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Aram Minas

Department of basic science, Facylty of Veterinary medicine,Urmia University, Urmia, Iran