IL8 mRNA as a potential blood-based biomarker in ulcerative colitis patients.

Background and aim: Early and timely diagnosis of inflammatory bowel disease IBD and timely still a major public health globally. However, lack of sensitivity and imprecision of the currently available biomarkers have impaired the ability to implement potentially effective therapies in a timely manner. The present study aimed to evaluate the clinical utility of Expression of IL-8 genes in diagnosis as well as assessment of IBD.Material and Methods: This case-control study was performed on 35 patients with IBD 20 ulcerative colitis and 15 crohn’s disease and 20 controls. For this purpose, the RNA of patients and control subjects was extracted and after cDNA synthesis, quantitative real time PCR qRT-PCR method was used to evaluate and express the target gene.Results: The results of this study showed that IL-8 expression in UC patients was significantly higher than control subjects p value: 0.026 . while the Comparison of IL-8 gene expression in CD subjects did not show a significant increase compared to controls.Conclusion: IL-8 gene can be used as a biomarker to distinguish between celiac, CD, and UC, as well as UC diagnosis from healthy subjects.