Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital

Aims The increasing usage of antibiotics can cause resistance to the treatment of infections,which can caused by bacteria, e.g. Pseudomonas aeruginosa.The aim of this study was totrace the class I, II and III integrons in isolates of P. aeruginosa of nosocomial infection anddetermining the antibiotic resistance pattern of the bacteria.Instrument & Methods In this cross-sectional study, 100 Pseudomonas aeruginosa clinicalisolates of infected wounds, bedsores, burns, urinary tract infections and respiratory tractinfections were collected from patients of 3 Isfahan City hospitals, Iran (Al Zahra, Kashani,Shariati) in 2015. After identification tests and antibiogram, integrons class I, II and III weredetected by M-PCR method.Data analysis were performed in SPSS 16 software using Chisquareand Fisher exact tests and the relationship between the presence of class III, II, I wascalculated by M-PCR test.Findings All isolates had multiple antibiotic resistances. The highest antibiotic resistancewas to Tetracycline (85%) and the lowest to Norfloxacin (12.5%). There were significantdifferences between class I and the two other classes of integrons (p=0.036). There was astatistically significant difference between the presence of blaTEM gene in Pseudomonasaeruginosa with other coding genes for antibiotic resistance (p=0.029).Conclusion Pseudomonas aeruginosa isolates are multi-drug resistant and almost all isolatesfrom clinical infections have class I, II and III Integrons.

کلیدواژه ها:

Pseudomonas aeruginosa ، Integrons ، Multiple Antibiotic Resistance ، Hospital Infections