HPV Prevalence Rates among 18-60-Year-Old Women with Normal Pap Smear Visiting Gynaecology Clinics in Isfahan University of Medical Sciences

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 418

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شناسه ملی سند علمی:

DTOGIMED03_089

تاریخ نمایه سازی: 26 بهمن 1398

چکیده مقاله:

Background and Aim : Cervical cancer is the fourth most common cancer that occurs in womenworldwide. In 2012 there were about 530,000 new cases reported of which about 270,000 were fatal. Thefraction of these deaths that could be attributed to Human Papilloma Virus (HPV) was estimated to be 100percent. Since the high-risk HPV virus causes cervical cancer, it is necessary to screen the population andeventually vaccinate against HPVMethods : This is a descriptive cross-sectional study conducted on 355 of the aforementioned 18-60-yearoldpatients with normal Pap smear (Conventional method). All women were examined clinically by agynecologist and for each case a new cervical smear was prepared using a plastic Ayer spatula and anendocervical brush. A second specimen of epithelial cells from transformation zone and endocervix wasprepared using a 360° sweep of nylon cytobrush. The collected specimens were placed in sterile tubescontaining 5 cc phosphate buffer saline, stored at -70°C for HPV molecular analysis. The DNA extractionfrom epithelial cells was conducted using the standard phenol-chloroform and the ethanol precipitationmethod. The extracted DNA was then stored at -20°C until tested for HPV subtypes (HPV subtypes 16, 18and 6 or 11) by PCR method. For quality control of DNA extraction from specimens, β-globin gene PCRwas done using PCO3/PCO4 primers, leading to the amplification of 110 bp segments of the β-globin gene,according to the previously described procedures [17,18]. The detection of HPV in the specimens was donewith GP5+ and GP6+primers in a PCR assay. HPV-DNA detection was performed by a standardGP5+GP6+PCRbased assay, which allows for detection of a broad spectrum of genital HPV types.Results : The results of the first step of PCR from the 355 tests conducted are presented in Table 1. Theyindicate the presence of HPV viruses in 25.35% of the cases. The frequency distribution of each type ofHPV virus in Pap smears is shown in Figure 1. Consistent with the fact that Types 16 and 18 have beenreported as high-risk, one of these types was found in 7.32% of the samples (26 out of 355 cases studiedhere). Half of the positive cases (45 out of 90) showed HPV types other than 16 and 18. These cases werenot included in this study.Conclusion : A study of prevalence of HPV was conducted on 355 women visiting gynecology clinics ofIsfahan University of Medical Sciences. The results show: 1. About 25% of all cases showed infection withHPV. 2. Of the infected cases, nearly half were of Type 16, 18, 6 or 11. 3. Types 16 and 18 were the mostcommon. 4. About half of the cases infected by HPV were other types 5. The administration of HPV vaccineis highly recommended as primary prevention of cervical cancer.

نویسندگان

Tagossadat Allameh

associate professor, fellowship of gynecology oncology , Isfahan University of Medical sciences

Tajossadat Allameh

associate professor, fellowship of gynecology oncology , Isfahan University of Medical sciences

Farinaz Farahbod

assistant professor fellowship of perinatology,Isfahan University of Medical sciences

Zahra Allameh

associate professor,obstetrician and gynecologist , Isfahan University of Medical sciences