The Effects of Chromium Supplementation on Gene Expression of Insulin, Lipid, and Inflammatory Markers in Infertile Women With Polycystic Ovary Syndrome Candidate for in vitro Fertilization: A Randomi

Background and Aim : This study was performed to determine the effects of chromium supplementationon the gene expression of insulin, lipid, and inflammatory markers in infertile women with (PCOS) whowere candidate for (IVF).Methods : Forty women, aged 18–40 years, who had been selected for IVF were recruited in thisrandomized double-blinded, placebo-controlled trial. They (n = 20/group) were randomly assigned intointervention groups to take either 200 μg/day of chromium or placebo for 8 weeks. Inflammatory markerswere measured at baseline and end of the trial. Genes related to insulin, lipid, and inflammation wereexpressed in peripheral blood mononuclear cells (PBMCs), using RT-PCR methodResults : Chromium supplementation led to a significant reduction in serum high sensitivity C-reactiveprotein (hs-CRP) (−1.4 ± 1.5 vs. + 0.2 ± 2.2 mg/L, p = 0.01) compared with the placebo. RT-PCR findingsindicated that chromium supplementation upregulated gene expression of peroxisome proliferator-activatedreceptor gamma (PPAR-γ) (p = 0.01), glucose transporter 1 (GLUT-1) (p = 0.001) and low-densitylipoprotein receptor (LDLR) (p = 0.01), as well as downregulated gene expression of interleukin-1 (IL-1)(p = 0.004) in PBMCs of patients with PCOS compared with the placebo. Chromium supplementation hadno significant effect on gene expression of IL-8, tumor necrosis factor alpha (TNF-α), transforming growthfactor beta (TGF-β) and vascular endothelial growth factor (VEGF).Conclusion : Overall, our findings demonstrated that infertile women with PCOS, who were candidate forIVF benefited from chromium supplementation for 8 weeks in terms of lowering hs-CRP and improving gene expression of PPAR-γ, GLUT-1, LDLR, and IL-1, though chromium had no effect on the geneexpression of IL-8, TNF-α, TGF-β, and VEGF.

کلیدواژه ها:

PCOS ، Chromium ، IVF