Association analysis of IL-12, IL-13 and TNF-α polymorphisms with susceptibility to brucellosis

  • سال انتشار: 1398
  • محل انتشار: بیستمین کنگره بین المللی میکروب شناسی ایران
  • کد COI اختصاصی: MEDISM20_355
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 420
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نویسندگان

Sima Kazemi

Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Asad Vaisi–Raygani

Department of Clinical Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Fariba Keramat

Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

Massoud Saidijam

Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Ali Reza Soltanian

Department of Biostatistics and Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran

Mahdi Alahgholi-Hajibehzad

Department of Immunology, Hamadan University of Medical Sciences, Hamadan, Iran

چکیده

Introduction & Objectives: The cytokine gene polymorphism is important for the genetic predisposition of infectious disease. The present study was aimed at identification the associations between IL-12 (+1188 A/C), IL-13 (-1512 A/C), IL-13 (-1112C/T) and TNF-α )-238 G/A) genotyping in Brucellosis patients. Materials & Methods: This case-control study was conducted on 107 Brucellosis patients and 107 healthy controls. The SNPs of TNF-α )-238 G/A) and IL-12 (+1188 A/C) were done by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and IL-13 genotyping at positions -1512 (A/C) and -1112 (C/T) were analysis by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) methods. Result: IL-13 (-1512A/C) was associated with Brucellosis risk in dominant model (OR (95% CI) =2.17 (1.02–4.62)), P-value=0.041. However, there was no difference in allele and genotype frequencies of TNF-α )-238 G/A), IL-12 (+1188 A/C) and IL-13 (-1112 C/T) between patients and controls. Conclusion: In conclusion the gene variation may be useful for identification of genotype as biomarkers for identification of people at high risk.

کلیدواژه ها

Cytokines; Polymorphisms; Brucellosis; Iran

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