The frequency of EBV in inflammatory lesions of periapical cysts using PCR techniques

Introduction and objective: One of the major problems of the periapical cyst is incomplete root canal treatment (incomplete endodontic treatment) that can be seen in some patients as holes in the gums using X-ray. Microorganisms not only play a role in the creation of apical lesions but also cause the diseases reaches the chronic stage. This study aimed to investigate the effect of Epstein-Barr virus (EBV) on the exacerbation of these diseases by using PCR techniques. Material and Methods: Twenty-one formalin fixed paraffin wax embedded tissue blocks of inflammatory periapical lesions, which were previously diagnosed, were gathered from patients who had referred to the Department of Pathology, Faculty of Dentistry of Islamic Azad university, Isfahan (Khorasgan) Branch during 2011-2018. DNA extraction from formalin fixed paraffin wax embedded tissue blocks was performed using Gene All extraction kit and Aaron gene. Extracted DNAs were confirmed using β-globin gene. In terms of EBV frequency, the samples were evaluated by Nested- PCR and PCR. Results: In the two experimental groups, the frequency of EBV was found respectively at 4 % of periapical cyst. Fisher’s test did not show a statistically significant difference (P value> 0.05). Conclusion: Based on the obtained results it can be claimed that EBV may possibly be involved in the pathogenesis or development of periapical lesions. Since limited researches have been done in this field and the results of the previous studies differ and apparently contradict each other, conducting further studies with larger sample sizes is recommended.

کلیدواژه ها:

Epstein-Barr virus (EBV) ، periapical cyst ، Nested- PCR and PCR.