The use of bacteriophage in aquatice disease treatment
- سال انتشار: 1398
- محل انتشار: بیستمین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM20_337
- زبان مقاله: انگلیسی
- تعداد مشاهده: 374
نویسندگان
Animal Science Research Department, Kerman Agricultural and Natural Resources Research and Education Center, Areeo, Kerman, Iran
Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
چکیده
Introduction and Objectives: Bacteriophages or phages are bacterial viruses that invade bacterial cells and, in the case of lytic phages, disrupt bacterial metabolism. Phage therapy is widely being reconsidered as an alternative to antibiotics. Bacterial resistance to antibiotics has now become a big issue, so the focus on the development of bacteriophage therapy has now increased. Replacing phage instead of antibiotics is a new option in the aquatic world. Materials and Methods: In this research, after separating bacteriophages of Aeromonas hydrophila with Chloroform and dual layer culture, bacteriophages was concentrated. After determining the minimum concentration of bacteria growth inhibition by bacteriophage under external conditions they were also used in in vivo and in vitro conditions or intra peritoneal injections and immersion. Results: The effect of bacteriophage on the survival rate of rainbow trout was when the bacterial concentration of 1 × 108 cfu and 1 × 103 pfu of the bacteriophage were immersed. There was no significant difference between the control and positive control groups in any of the treatments, but there was a significant difference between the control group and the treatments (intra peritoneal injections and immersion) (P < 0.05). Data analysis also showed this The results of this study showed that one of the ways to increase the survival rate of trout fish was to increase the survival rate of trout fish and reduce the mortality rate of 1× 108ml-1 of bacteria to direct contact with bacteria and bacteriophage in the treatment group had the most effect on the survival rate of trout and eventually reduced mortality. Conclusion: The results of this study showed that bacteriophages are one of the ways to increase the survival rate of trout fish.کلیدواژه ها
Aeromonas hydrophila, Bacteriophage, survival rateاطلاعات بیشتر در مورد COI
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