Prevalence of Helicobacter pylori and Staphylococcus aureusin human gastric biopsy specimens

Introduction and objectives: The human stomach is anintolerable microniche ecosystem hosting wide range of persistent bacteria. Regardless the Helicobacter pylori(H. pylori), there are some clue that Staphylococcus aureus(S. aureus) can also colonized at this site (1). In current study, we aim to investigate the exact prevalence of these two major bacteria in human gastric biopsies. Materials and methods: In this study, stomach biopsy samples were collected from patients withgastric disease referring to the gastroenterology section at Mehrad Hospital and Labafi-nejad Hospital, Tehran, Iran. The biopsies obtained in tubescontaining Thioglycollate(4°C) will be shippedto the laboratory for further bacterial analysis. DNA extraction using a commercial genomic extraction kit was conducted before PCR approach. The clinical control samples were also used as positive control in this study. Results: We analyzed the gastric biopsy samples from 45 patients who presented for endoscopy at Mehrad Hospital and Labafi-nejad Hospital. The patients were 45(71%) and 13 (28.8%) men and women, respectively, with a median age of 48 years (range, 16 to 80 years). In this study, we have identified bacterial species from gastric biopsies of 12 H. pylori-positive and 16 H. pylori-negative suffering from gastric diseases (95% Cl : 1.03 – 5.1,p value : 0.04)& 9 S.aureus positive and 19 S.aureus negative suffering from gastric diseases (95% Cl: 0.5 – 5.8, pvalue: 0.3). No association was found between age, gender and certain bacterial colonization. Conclusion: Current body of evidences in the field of gastrointestinal tract research are suggesting that any effective antibiotic therapy against H. pylori should have considered co-existence of S. aureus. Further molecular analysis using biopsy samples are needed before final conclusion about current drawn idea.

کلیدواژه ها:

H. pylori ، Staphylococcus aureus ، PCR ، colonization