Frequency of PMQR genes of isolated Pseudomonas aeruginosa strains from patients in Kerman hospitals, Iran

Introduction and Objectives: Emerging antibiotic resistance is a major worldwide threat to human’s health. Understanding and fighting in this direction requires a full assessment of the current status on the prevalence of antibiotic resistance genes and understanding their correlation with each other. Plasmid mediated quinolone resistance (PMQR) genes of Pseudomonas aeruginosa strains have arisen as a significant concern in recent years. Accordingly, the aim of the present research was to determine the frequency of PMQR genes related to the isolates of P. aeruginosa obtained from clinical samples of Kerman hospitals located in south east of Iran. Material and Methods: In this sectional study, clinical samples were used to isolate different strains of P. aeruginosa. Accordingly, in the first step, antibiotic resistance was evaluated using Kirby-Bauer and CLSI criteria. In the second step, following DNA extraction, qnr and aac (6)-Ib-cr genes were amplified using PCR method and results were analyzed. Results: Cefexim (80%), Nalidixic acid (75%) showed maximum antibiotic resistance and imipenem (25%), ciprofloxacin (35%) had the minimum antibiotic resistance. 10 samples of qnr A (16.66%) and 8 samples of qnrB (13.33%) as well as 7 samples of qnrS (11.66%) were positive and 5 samples of aac(6)-Ib-cr (8/33%) were positive. Conclusion: Using molecular methods for diagnosis of pathogenic bacteria such as P. aeruginosa and rapid diagnosis of their resistant genes has proved to be very important and critical. On the other hand, it is necessary to monitor for the spread of PMQR genes of clinical isolates and to ensure careful antibiotic use in a hospital setting.

کلیدواژه ها:

Pseudomonas aeruginosa ، antibiotic resistance ، PMQR genes frequency ، qnr ، aac(6)-cr ، PCR.